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Catalog Number
18038240
Unit Size
5,000 units
Price (USD)
1,529.65 Online price
1,952.00
Availability
-
Quantity
Quantity
Quantity
Quantity
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Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity (see figure). With Taq DNA Polymerase, you get:
Your choice of recombinant or native enzyme Amplification of PCR products up to 5 kb in size An enzyme that is licensed and qualified for PCR
Applications
Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing
Source
Native enzyme is purified from Thermus aquaticus YT1.
Unit definition
One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
Applications
Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing
Source
Native enzyme is purified from Thermus aquaticus YT1.
Unit definition
One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection Method
Primer-probe
Format
Stand-alone enzyme
GC-Rich PCR Performance
Low
PCR Method
qPCR
Polymerase
Taq DNA Polymerase
Reaction Speed
Standard
Exonuclease Activity
5' - 3'
Product Type
DNA Polymerase
Quantity
5000 Units
Shipping Condition
Approved for shipment on Wet or Dry Ice
Concentration
5 U/μL
Fidelity (vs. Taq)
1 X
Hot Start
No
Overhang
3'-A
Reaction Format
Separate Components
Size (Final Product)
5 kb or less
Starting Material
DNA
Contents & Storage
Contains:
• 1000 μl Taq DNA Polymerase (5 U/μl)
• 20 ml 10X PCR buffer (without magnesium)
• 10 ml Magnesium Chloride (50 mM)
Store at -20°C in a non-frost-free freezer. Stable for 6 months when stored properly.
• 1000 μl Taq DNA Polymerase (5 U/μl)
• 20 ml 10X PCR buffer (without magnesium)
• 10 ml Magnesium Chloride (50 mM)
Store at -20°C in a non-frost-free freezer. Stable for 6 months when stored properly.
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