Can regular Klenow be used for BioPrime Plus Array CGH labeling rather than exo-Klenow?
Regular Klenow may be used but is not recommended because yield, sensitivity, and reproducibility may be compromised compared to exo-Klenow. Exo-Klenow polymerase lacks both 5'-3#146; and 3#146;-5#146; exonuclease activity, producing higher yields of labeled sample than standard Klenow and thereby increasing sensitivity. Exo-Klenow polymerase also incorporates modified nucleotides more effectively than standard Klenow, enabling you to obtain stronger hybridization intensities and greater reproducibility of results. Another advantage with a simplified random priming protocol is that the protocol takes less than three hours.