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MAX Efficiency® DH5α™ Competent Cells

Catalog number: 18258012

Invitrogen™  Related applications: Transformation

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Description

MAX Efficiency® DH5α™ Competent Cells are a well-known, versatile strain that can be used in many everyday cloning applications. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α™ cells increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

Features of MAX Efficiency® DH5α™ Competent Cells include:

• Transformation efficiency up to 1 × 109 transformants/µg plasmid DNA
• High plasmid yield from the DH5α™ (endA1) E. coli strain
• Blue/white screening capable (lacZΔM15)
• Greater insert stability (recA1)

About MAX Efficiency® DH5α™ Competent Cells
MAX Efficiency® DH5α™ Competent Cells have been prepared by a proprietary modification of the procedure of Hanahan (1983). These cells are suitable for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors. The Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors, and can therefore be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. DH5α™ cells are capable of being transformed efficiently with large plasmids, and can also serve as a host for the M13mp cloning vectors if a lawn of DH5α-FT™, DH5αF′™, DH5αF′IQ™, JM101, or JM107 is provided to allow plaque formation.

Genotype: F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk-, mk+) phoA supE44 λ-thi-1 gyrA96 relA1

Find the strain and format that you need
DH5α™ cells are available with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats and in single-use aliquots.

Subcloning Efficiency™ DH5α™ Competent Cells—our most economical competent cell for everyday use
Library Efficiency® DH5α™ Competent Cells—economical and for use with difficult-to-transform DNA
MAX Efficiency® DH5α™-T1R Competent Cells—T1 phage resistant, >109 transformants/µg plasmid DNA
One Shot® MAX Efficiency® DH5α™-T1R Competent Cells—single-use format, T1 phage resistant, >109 transformants/µg plasmid DNA
ElectroMAX™ DH5α™ Competent Cells—electrocompetent with >1010 transformants/µg plasmid DNA and ideal for cDNA libraries or larger inserts
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Chemically Competent
Species: E. coli
Format: Tube(s)
Transformation Efficiency: > 1x10^9
Bacterial or Yeast Strain: DH5α™
Product Size: 10 reactions
Blue-White Screening: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Reduces Recombination: Yes
Cloning Methylated DNA: No
Improves Plasmid Quality: Yes
Propagating ccdB Vectors: Not for ccdB vector propagation
F' Episome (to make ssDNA): Lacks F' Episome
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA): No
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Shipping Condition: Dry Ice

Contents & storage

Contains:
• Max Efficiency® DH5α™ Competent Cells: 5 vials, 200 µl each (total of 1 ml)
• pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
• S.O.C. medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

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Manuals & protocols

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