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Invitrogen™

MAX Efficiency™ DH5α Competent Cells

Catalog number: 18258012
Invitrogen™

MAX Efficiency™ DH5α Competent Cells

Catalog number: 18258012
Catalog Number
18258012
Unit Size
5 x 200 µL
Price (USD)
246.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
182580125 x 200 µL
246.00
Your Price:
***
Product Overview
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MAX Efficiency DH5α Competent Cells are a well-known, versatile strain that can be used in many everyday cloning applications. In addition to supporting blue/white screening, recA1 and endA1 mutations in DH5α cells increase insert stability and improve the quality of plasmid DNA prepared from minipreps.

Features of MAX Efficiency DH5α Competent Cells include:

• Transformation efficiency up to 1 × 109 transformants/µg plasmid DNA
• High plasmid yield from the DH5α (endA1) E. coli strain
• Blue/white screening capable (lacZΔM15)
• Greater insert stability (recA1)

About MAX Efficiency DH5α Competent Cells
MAX Efficiency DH5α Competent Cells have been prepared by a proprietary modification of the procedure of Hanahan (1983). These cells are suitable for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors. The Φ80lacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors, and can therefore be used for blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal. DH5α cells are capable of being transformed efficiently with large plasmids, and can also serve as a host for the M13mp cloning vectors if a lawn of DH5α-FT, DH5αF′, DH5αF′IQ, JM101, or JM107 is provided to allow plaque formation.

Genotype: F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk-, mk+) phoA supE44 λ-thi-1 gyrA96 relA1

Find the strain and format that you need
DH5α cells are available with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats and in single-use aliquots.

Subcloning Efficiency DH5α Competent Cells—our most economical competent cell for everyday use
Library Efficiency DH5α Competent Cells—economical and for use with difficult-to-transform DNA
MAX Efficiency DH5α-T1R Competent Cells—T1 phage resistant, >109 transformants/µg plasmid DNA
One Shot MAX Efficiency DH5α-T1R Competent Cells—single-use format, T1 phage resistant, >109 transformants/µg plasmid DNA
ElectroMAX DH5α Competent Cells—electrocompetent with >1010 transformants/µg plasmid DNA and ideal for cDNA libraries or larger inserts
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Competent Cell
Contains F' Episome
Lacks F' Episome
Efficiency
High Efficiency (>10^9 cfu/µg)
Cell Type
Chemically Competent
Improves Plasmid Quality
Yes
Cloning Methylated DNA
No
Transformation Efficiency Level
High Efficiency (> 10^9 cfu⁄µg)
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Bacterial or Yeast Strain
DH5α
Blue/White Screening
Yes
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
Yes
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
No
Species
E. coli
Format
Tube
Product Line
DH5a, MAX Efficiency™
For Use With (Application)
Cloning

Contents & Storage

Contains:
• Max Efficiency DH5α Competent Cells: 5 vials, 200 µl each (total of 1 ml)
• pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
• S.O.C. medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

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