Thermo Scientific™

CaptureSelect™ Follicle Stimulating Hormone (FSH) Affinity Matrix

Catalog number:  19431801L

Related applications:

Antibody Purification

| Protein Purification & Isolation

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Description

CaptureSelect™ Follicle Stimulating Hormone (FSH) Affinity Matrix has been specifically designed for the purification of FSH from recombinant sources.

Features of this affinity matrix include:

• Highly selective for intact, recombinant FSH, binding the alpha/beta chain interface
• Efficient purification of recombinant FSH with high yields and retained activity
• Excellent scalability
• Non-animal-derived

Recombinant FSH, also known as follitropin, is a hormone regulating the development, growth, pubertal maturation, and reproductive processes of the body. The high selectivity and yields obtained using CaptureSelect™ Follicle Stimulating Hormone Affinity Matrix and its unique ability to only bind intact FSH enable a robust and efficient purification process with excellent purity obtained in one step.

Free of Animal Components
Our CaptureSelect™ products are affinity ligands created by a proprietary technology based on Camelid-derived single domain antibody fragments. The ligand is a 14 kDa single domain fragment comprising the 3 CDRs that form the antigen binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main Characteristics:

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect™ FSH affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: ~3 mg/mL resin
Elution conditions (neutral pH): 20 mM Tris, 2.0 M MgCl2, pH 7.0-7.5
Elution conditions (acidic pH): glycine, pH 3.0
Flow characteristics: 200–400 cm/h (up to 1 bar)
Formulation buffer: 20%(v/v) ethanol
Caution: For manufacturing, processing, or repacking.

Specifications

Column Type: Bulk Resin
Format: Bottle(s)
Immobilized Phase: Anti FSH
Product Line: CaptureSelect™
Product Size: 1 L
Support Matrix: Agarose based, aldehyde activated

Contents & storage

Store in refrigerator (2–8°C).