Thermo Scientific™

CaptureSelect™ hCG Affinity Matrix

Catalog number:  194341005

Related applications:

Chromatography

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Description

The CaptureSelect™ hCG Affinity Matrix was specifically designed for the purification of human chorionic gonadotropin (hCG) from recombinant sources.

Some features of the CaptureSelect hCG Affinity Matrix include:
• Selective for hCG, with cross-binding to LH, FSH, and TSH
• Enables efficient purification of recombinant gonadotropins with high yields and purity
• Offers excellent scalability
• Non–animal-derived

Human gonadotropins (hCG, LH, FSH, TSH) are glycoprotein hormones secreted by gonadotrope cells of the pituitary gland that are often produced as recombinant proteins. They have an identical alpha chain subunit that is recognized by the ligand. Consequently, the CaptureSelect hCG Affinity Matrix shows binding to all human gonadotropins. The high selectivity and yields obtained using CaptureSelect hCG Affinity Matrix enable a robust and efficient purification process for hCG with excellent purity obtained in one step.

Free of animal components
CaptureSelect affinity ligands are created with a proprietary technology based on camelid-derived single domain antibody fragments. The ligand is a 13 kDa single domain fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics

Matrix: agarose-based, aldehyde-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect hCG affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: approx. 4 mg/mL resin (determined with HCG)
Elution conditions: 20 mM citric acid, pH 3.0
Flow characteristics: 200–400 cm/h (up to 1 bar)
Formulation buffer: 20% (v/v) ethanol
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Column Type: Agarose Resin
Shipping Condition: Room Temperature
Product Line: CaptureSelect™
Format: Agarose Bead
Stationary Phase: Anti Gonadotropin

Contents & storage

Store in refrigerator (2–8°C).