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CaptureSelect™ CH1-XL Affinity Matrix
Thermo Scientific™

CaptureSelect™ CH1-XL Affinity Matrix

Thermo Scientific™ CaptureSelect™ CH1-XL Affinity Matrix is the improved version of CaptureSelect IgG-CH1 Affinity Matrix.
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Catalog NumberQuantity
194346205050 mL, 50 mL, 50 mL, 50 mL
19434620055 mL, 5 mL, 5 mL, 5 mL
194346201010 mL, 10 mL, 10 mL, 10 mL
1943462250250 mL, 250 mL, 250 mL, 250 mL
194346201L1 L, 1 L, 1 L, 1 L
194346205L5 L, 5 L, 5 L, 5 L
194346210L10 L, 10 L, 10 L, 10 L
Catalog number 1943462050
Price (USD)
6,980.00
Each
Quantity:
50 mL, 50 mL, 50 mL, 50 mL
Price (USD)
6,980.00
Each

CaptureSelect CH1-XL Affinity Matrix is the improved version of CaptureSelect IgG-CH1 Affinity Matrix. The specificity is the same, but it features a wider elution range (pH 3.0-4.5), increased binding capacity, and improved stability at higher pH.

CaptureSelect CH1-XL Affinity Matrix recognizes the CH1 domain of human IgG antibodies, enabling purification of all subclasses (1, 2, 3, and 4) of human IgG and Fab fragments, independent of the light-chain isotype and source material.

CaptureSelect CH1-XL Affinity Matrix features include:

  • Platform approach for purification of IgGs and their Fab fragments directly from complex source materials in a single step with high purity and yield
  • Elution possible at pH 4.0–4.5 with 50 mM acetate buffer
  • No co-purification of overproduced free light chain due to selectivity for the CH1 domain
  • No binding to bovine antibodies, so presence of fetal bovine serum is not a problem
  • Excellent scalability
  • Non-animal derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

  • Matrix: agarose-based, epoxide-activated
  • Average particle size: 65 μm
  • Ligand: CaptureSelect CH1-XL affinity ligand
  • Ligand coupling method: epoxide coupling
  • Binding capacity: >15 mg/mL human Fab fragment depending on flow rate, column height, contact time. For humanized Fabs lower capacities can be observed.
  • Elution conditions: 100 mM glycine buffer, pH 3.0; 50 mM acetate buffer, pH 4.0–4.5
  • Flow rate: 50–200 cm/h (up to 2 bar)
  • Formulation buffer: 20% (v/v) ethanol
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Binding Capacity>15 mg/mL, >15 mg/mL, >15 mg/mL, >15 mg/mL
Flow Rate50 to 200 cm/hr. (Up to 2 bar), 50 to 200 cm/hr. (Up to 2 bar), 50 to 200 cm/hr. (Up to 2 bar), 50 to 200 cm/hr. (Up to 2 bar)
Ligand TypeCH1-XL affinity ligand, CH1-XL affinity ligand, CH1-XL affinity ligand, CH1-XL affinity ligand
MatrixAgarose-based, epoxide-activated, Agarose-based, epoxide-activated, Agarose-based, epoxide-activated, Agarose-based, epoxide-activated
Particle Size65 μm
Quantity50 mL, 50 mL, 50 mL, 50 mL
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice, Approved for shipment at Room Temperature or on Wet Ice, Approved for shipment at Room Temperature or on Wet Ice, Approved for shipment at Room Temperature or on Wet Ice
Stationary PhaseAnti-IgG-CH1, Anti-IgG-CH1, Anti-IgG-CH1, Anti-IgG-CH1
FormAgarose Bead, Resin Slurry, Agarose Bead, Resin Slurry
Particle Size65 μm
Product LineCaptureSelect, CaptureSelect, CaptureSelect, CaptureSelect
TypeAffinity Matrix
Unit SizeEach
Contents & Storage
Store at 2°C to 8°C.