Thermo Scientific™

CaptureSelect™ CH1-XL Affinity Matrix

Catalog number: 1943462250
Thermo Scientific™

CaptureSelect™ CH1-XL Affinity Matrix

Catalog number: 1943462250
Catalog Number
1943462250
Unit Size
250 mL
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5 mL
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CaptureSelect CH1-XL Affinity Matrix is the improved version of CaptureSelect IgG-CH1 Affinity Matrix. The specificity is the same, but it features a wider elution range (pH 3.0–4.5), increased binding capacity, and improved stability at higher pH.

CaptureSelect CH1-XL Affinity Matrix recognizes the CH1 domain of human IgG antibodies, enabling purification of all subclasses (1, 2, 3, and 4) of human IgG and Fab fragments, independent of the light-chain isotype and source material.

CaptureSelect CH1-XL Affinity Matrix features include:

  • Platform approach for purification of IgGs and their Fab fragments directly from complex source materials in a single step with high purity and yield
  • Elution possible at pH 4.0–4.5 with 50 mM acetate buffer
  • No co-purification of overproduced free light chain due to selectivity for the CH1 domain
  • No binding to bovine antibodies, so presence of fetal bovine serum is not a problem
  • Excellent scalability
  • Non-animal derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

  • Matrix: agarose-based, epoxide-activated
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect CH1-XL affinity ligand
  • Ligand coupling method: epoxide coupling
  • Binding capacity: >15 mg/mL human Fab fragment depending on flow rate, column height, contact time. For humanized Fabs lower capacities can be observed.
  • Elution conditions: 100 mM glycine buffer, pH 3.0; 50 mM acetate buffer, pH 4.0–4.5
  • Flow rate: 50–200 cm/h (up to 2 bar)
  • Formulation buffer: 20% (v/v) ethanol

Specifications

Binding Capacity
>15 mg/mL
Flow Rate
50 to 200 cm/hr. (Up to 2 bar)
Matrix
Agarose-based, epoxide-activated
Shipping Condition
Approved for shipment at Room Temperature or on Wet Ice, Wet Ice
Particle Size
65 μm
Form
Agarose Bead
Product Line
CaptureSelect™
Quantity
250 mL
For Use With (Application)
IgG purification
Target
CH1 domain of IgG
Type
Affinity Matrix
Ligand Type
CH1-XL affinity ligand
Stationary Phase
Anti-IgG-CH1
Format
Bottle

Contents & Storage

Store at 2°C to 8°C.

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