Thermo Scientific™

CaptureSelect™ Factor X Affinity Matrix

Catalog number: 194370201L
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Thermo Scientific™

CaptureSelect™ Factor X Affinity Matrix

Catalog number: 194370201L
Have Questions?
Catalog Number
194370201L
Unit Size
1 L
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Unit Size
5 mL
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10 mL
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50 mL
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250 mL
Price (USD)
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-
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-
Catalog Number
Unit Size
5 L
Price (USD)
Availability
-
Quantity
-
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
194370201L1 L
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29437020055 mL
-
294370201010 mL
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294370205050 mL
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1943702250250 mL
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194370205L5 L
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CaptureSelect Factor X Affinity Matrix was specifically designed for the purification of human Factor X from plasma and recombinant sources.

Features of the CaptureSelect Factor X Affinity Matrix include:

  • Highly selective for human Factor X with no affinity for other human plasma proteins
  • Binds both Factor X and Factor Xa in the presence of calcium
  • Enables efficient purification of Factor X with high yields and retained activity
  • Mild elution at neutral pH using EDTA
  • Offers excellent scalability
  • Non-animal-derived

Coagulation Factor X is a vitamin K-dependent, liver-produced serine protease that serves as the first enzyme in the coagulation cascade to form fibrin. It is a two-chain glycoprotein with a molecular weight of approximately 59 kDa. Factor X normally circulates in the plasma as an inactive molecule with activation of Factor X involved in both the intrinsic and extrinsic coagulation pathways.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics

  • Matrix: agarose-based
  • Average particle size: 65 ± 10 μm
  • Ligand: CaptureSelect Factor X affinity ligand
  • Ligand coupling method: epoxide
  • Binding capacity: >10 mg/mL resin
  • Binding conditions: 50 mM Tris, 150 mM NaCl, 5 mM CaCl2, pH 7
  • Elution conditions: 50 mM Tris, 150 mM NaCl, 5 mM EDTA, pH 7
  • Flow characteristics: 50–200 cm/h (up to 2 bar)
  • Formulation buffer: 20% (v/v) ethanol

Specifications

Binding Capacity
>10 mg/mL
Flow Rate
50 to 200 cm/hr. (Up to 2 bar)
Matrix
Agarose-based
Particle Size
65 ± 10 μm
Form
Resin Slurry
Product Line
CaptureSelect™
Quantity
1 L
Type
Affinity Matrix
Ligand Type
Factor X affinity ligand
Stationary Phase
Factor X

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