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Self Pack® Empty Column (4.6 x 100 mm, 1.7 mL) - FAQs

View additional product information for Self Pack® Empty Column (4.6 x 100 mm, 1.7 mL) - FAQs (1954126)

2 product FAQs found

How many histidines must be present in my purified protein when using the ProBond Purification System?

We have purified proteins with a minimum of 6 histidines. Some literature has indicated purification from as little as 4 or 5 histidines.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How does the ProBond Purification System work?

The ProBond resin is coupled with iminodiacetic acid groups (IDA). IDA is loaded with Ni2+ ions and binds Ni2+ by three coordination sites. The resin is used to purify 6x His-tagged overexpressed proteins. One mL should bind at least 1 mg of recombinant protein.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.