SuperScript® Plasmid System with Gateway®Technology and ElectroMAX™ DH10B™ Competent Cells

Catalog number: 19625011

Invitrogen™  Related applications: cDNA Libraries & Library Construction

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Description

The SuperScript® Plasmid System is designed for synthesis of double-stranded cDNA from a purified mRNA population. The cDNA is suitable for directional cloning into the pSPORT1 vector or the pCMV

• SPORT6 plasmid vector for subsequent generation of cDNA libraries and use of Gateway® Technology. The SuperScript® Plasmid System:
• Results in greater cDNA yields and increased full-length cDNAs with SuperScript® II RT
• Offers a simplified primer-adapter strategy for directional cloning
• Offers one-tube format for first- and second-strand reactions, improving cDNA yields
• Fractionates cDNA using efficient and convenient pre-packed columns
• Ligates inserts into a pre-cut Not I-Sal I vector prepared to minimize background
For Research Use Only. Not for use in diagnostic procedures.

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Specifications

Product Size: 1 set

Contents & storage

The SuperScript® Plasmid System with Gateway® Technology includes components listed above. Store all cDNA cloning and ligation reagents at -20°C. Store cDNA size fractionation columns at +4°C.

Documents

Manuals & protocols