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Pierce™ TCEP-HCl, 10 g - FAQs

Pierce™ TCEP-HCl, 10 g - FAQs

View additional product information for Pierce™ TCEP-HCl, No-Weigh™ Format - FAQs (A35349, 20491, 20490)

2 product FAQs found

Are other TCEP-based products available besides Immobilized TCEP Disulfide Reducing Gel?

Yes. We offer TCEP-HCl (1g solid, Cat. No. 20490, 10g solid, Cat. No. 20491), TCEP-HCl 10 x 1 mg No-weigh format, Cat. No. A35349 and TCEP Solution, Neutral pH (5 mL of stable 500 mM TCEP, Cat. No. 77720).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can other reducing agents other than DTT or BME be used to reduce proteins prior to electrophoresis? For example, what about TCEP (Tris Carboxy Ethyl Phosphene)?

TCEP, Tris Carboxy Ethyl Phosphene is an alternative sulfhydryl reducing agent for protein samples. It is an extremely potent and effective reducing agent for particularly ‘difficult' proteins. It is compatible with the Tris-Glycine gels and NuPAGE gels. It should be added to the sample buffer for these systems. 20 mM final (maximum) concentration is sufficient for samples. You may add alkylating agents, e.g. Iodine (50 mM Iodoacetic acid), to prevent re-forming of S-S bonds but it is not necessary. Do not heat because this will hydrolyze much of your sample. Instead let the sample sit for several minutes at RT and then load.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.