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Gibco™

DMEM, high glucose, no glutamine, no methionine, no cystine

Catalog number: 21013024
Have Questions?
Gibco™

DMEM, high glucose, no glutamine, no methionine, no cystine

Catalog number: 21013024
Have Questions?
Catalog Number
21013024
Unit Size
500 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
21013024500 mL
-
Product Overview
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Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This DMEM is modified as follows:
WithWithout
• High Glucose• L-glutamine
• Phenol Red• Sodium Pyruvate
 • HEPES
 • L-methionine
 • L-cystine


The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Specifications

Amino Acids
No Cystine, No Methionine
Cell Line
HeLa, 293, Cos-7, and PC-12
Cell Type
Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Classification
Animal Origin-free
Concentration
1 X
Form
Liquid
Product Type
DMEM (Dulbecco's Modified Eagle Medium)
Serum Level
Standard Serum Supplementation
Shelf Life
12 Months From Date of Manufacture
Sterility
Sterile-filtered
With Additives
High Glucose, Phenol Red
Without Additives
No Glutamine, No HEPES, No Sodium Pyruvate, No Methionine, No Cystine
Green Features
Sustainable packaging
Manufacturing Quality
cGMP-compliant under the ISO 13485 standard
Product Line
Gibco™
Quantity
500 mL
Shipping Condition
Room Temperature

Contents & Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

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