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Thermo Scientific™

Pierce™ Gentle Ag/Ab Binding Buffer, pH 8.0

Catalog number: 21020
Thermo Scientific™

Pierce™ Gentle Ag/Ab Binding Buffer, pH 8.0

Catalog number: 21020
Catalog Number
21020
Unit Size
1 L
Price (USD)
Price: 156.00
Your Price:
Availability
-
Quantity
Catalog Number
Unit Size
3.75 L
Price (USD)
Price: 305.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
210201 L
Price: 156.00
Your Price:
-
210123.75 L
Price: 305.00
Your Price:
-

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Product Overview
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Additional Information
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Thermo Scientific Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads. The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.

Shared features of all Thermo Scientific Pierce IgG Binding and Elution Buffers:

Ready to use—carefully formulated and prefiltered 1X solutions
Three binding buffers—optimized for Protein A, Protein G or Protein A/G
Two elution buffer options—acidic (pH 2.8) or high salt (near-neutral)
Versatile—elution buffers are appropriate for antibody-antigen affinity purification

Products available for each antibody purification method:
Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA
Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA
Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide
IgG Elution Buffer: pH 2.8; amine-based
Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide
Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)

Related Products
Pierce™ Gentle Ag/Ab Elution Buffer, pH 6.6
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Description
0.25 in. x 4.6mm I.D. Frit
Quantity
1 L
Reagent Type
Binding Buffer
Format
Liquid
Product Line
Pierce™
Product Type
Binding Buffer
pH
8

Contents & Storage

4°C