Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO₂ equilibration.
The complete formulation is available.

Using L-15
L-15 contains no proteins, lipids, or growth factors. Therefore, L-15 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

cGMP manufacturing and quality system
Leibovitz's L-15 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.