Pierce™ Biotinylated Protein Interaction Pull-Down Kit

Katalognummer	Menge
21115	1 Kit

Katalognummer 21115

Preis (EUR)

702,00

Zum Warenkorb hinzufügen

Menge:

1 Kit

Großbestellung oder individuelle Größe anfordern

Preis (EUR)

702,00

Zum Warenkorb hinzufügen

Das Thermo Scientific Pierce Pulldown-Kit für biotinylierte Proteininteraktionen enthält alle notwendigen Komponenten zum Einfangen und Reinigen von Interaktoren eines biotinmarkierten Proteins oder Liganden.

Merkmale des Pulldown-Kits für biotinylierte Proteininteraktionen:

• Bietet eine vollständige, kostengünstige und anwenderfreundliche Strategie für die Erkennung von Protein-Protein-Interaktionen
• Nutzt gängige Laborgeräte und Reagenzien (z. B. Mikrozentrifugen, Minigele, Proteinfärbungen)
• Anpassbar an Anforderungen für Einzel- oder Mehrfachproben
• Flexibles Pulldown-Format mit Spinbechern für die einfache und effiziente Verarbeitung von Streptavidin-Agarose-Beads

Sie stellen biotinylierte Protein als ‚Köder‘ zur Verfügung (siehe

Ähnliche Produkte
EZ-Link™ Desthiobiotinylierungs- and Pulldown-Kit

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

AssayAffinitätsreinigung

Zur Verwendung mit (Anwendung)Pull-Down (Biotin-basiert)

Zur Verwendung mit (Geräte)Mikrozentrifuge

FormatKit

Menge1 Kit

Ausreichend für25 Reaktionen

ZielNicht zielspezifisch

ProduktliniePierce

TypPull-Down-Kit

Unit SizeEach

Inhalt und Lagerung

Ausreichend für: Mindestens 25 Pulldown-Reaktionen mit jeweils 25 µl Harz
• Streptavidin-Agaroseharz, 1,5 ml
• TBS-Packung (ergibt 500 ml), 1 Packung
• Biotin-Blockierungslösung, 15 ml
• Waschpuffer (pH-Wert 5,0), 100 ml
• Elutionspuffer (pH-Wert 2,8), 50 ml
• Pierce Spin-Säulen und Zubehör, 27 Säulen
• Entnahmeröhrchen für Mikrozentrifugen, 200 Röhrchen

bei 4 °C lagern

Häufig gestellte Fragen (FAQ)

If my protein is endogenously expressed, which Thermo Scientific Protein:Protein Interaction (PPI) kit should I choose?

We recommend the biotin kit for endogenously expressed proteins. The reason for this is the wide variety of biotinylation reagents that can be used to label the detection protein of the binding pair. For help choosing a biotinylation reagent, refer to the Biotinylation Selection Guide available at our website.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How is the tagged protein detected on the membrane using the Thermo Scientific Protein:Protein Interaction (PPI) kits?

The biotin kit utilizes streptavidin-HRP to detect the biotinylated protein bound to the prey protein on the membrane. The GST kit utilizes a polyclonal goat anti-GST antibody for detection of the glutathione-S-transferase tagged fusion protein bound to another protein on a membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How many reactions can be performed using one Thermo Scientific Protein:Protein Interaction (PPI) kit?

The Thermo Scientific Pull-Down Protein:Protein Interaction Kits contain a complete, validated set of reagents specifically developed for performing pull-down assays.The kits contain sufficient materials for conducting 25 pull-down assays.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are all of the conditions for binding the prey protein to the bait protein described in the Thermo Scientific Protein:Protein Interaction (PPI) kits?

No. Every protein pair will require unique conditions in order to bind with one another. Factors to consider are pH, ionic strength of the buffer, need for cofactors, whether the interaction is long term or transient, etc. The researcher is recommended to perform a literature search or try several different conditions in order to determine what conditions are best for the binding of their proteins of interest. The kit includes a “universal“ wash buffer formed by diluting the lysis buffer 1:1 in TBS.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

With the Thermo Scientific Protein:Protein Interaction (PPI) kits, what methods can be used to detect the prey protein?

The prey protein can be detected following transfer on a membrane. The membrane procedures uses 1:100,000 - 1:500,000 dilution of the anti-GST antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Pierce™ Biotinylated Protein Interaction Pull-Down Kit FAQs