Pierce™ Biotinylated Protein Interaction Pull-Down Kit

Catalog number: 21115

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific Pierce Biotinylated Protein Interaction Pull-Down Kit contains the necessary components to capture and purify interactors of a biotin-labeled protein or ligand.

Features of the Biotinylated Protein Interaction Pull-Down Kit:

• Provides a complete, affordable and easy-to-use strategy for discoverying protein:protein interactions
• Uses common laboratory equipment and reagents (e.g., microcentrifuges, mini-gels, protein stains)
• Adaptable to single- or multiple-sample demands
• Flexible pull-down format uses spin cups for easy and efficient manipulation of streptavidin agarose beads

You provide the biotinylated protein as the "bait" (see

Related Products
EZ-Link™ Desthiobiotinylation and Pull-Down Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Technique: Pull-Down (Biotin-Based)
Assay Type: Affinity Purification
Product Size: 25 reactions
Target Specificity: Not Target-Specific
For Use With (Equipment): Microcentrifuge

Contents & storage

Sufficient For: At least 25 pull-down reactions, each using 25 µL of resin
• Streptavidin Agarose Resin, 1.5 mL
• TBS Pack (makes 500mL), 1 pack
• Biotin Blocking Solution, 15 mL
• Wash Buffer (pH 5.0), 100 mL
• Elution Buffer (pH 2.8), 50 mL
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 200 tubes

Store at 4°C

Documents

Manuals & protocols

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