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Ham's F-12K (Kaighn's) Medium

Green features
Catalog number: 21127022
Gibco™

Ham's F-12K (Kaighn's) Medium

Green features
Catalog number: 21127022
Catalog Number
21127022
also known as 21127-022
Unit Size
500 mL
Price (USD)
32.84
Your Price:
Availability
***
Quantity
Catalog Number
21127030
also known as 21127-030
Unit Size
10 x 500 mL
Price (USD)
283.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
21127022
also known as 21127-022
500 mL
32.84
Your Price:
***
21127030
also known as 21127-030
10 x 500 mL
283.00
Your Price:
***
Product Overview
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Ham's F-12K (Kaighn's) Medium is a modification of Ham's F-12 Nutrient Mixture. Ham's F-12K (Kaighn's) Medium was developed for primary human hepatocytes, as well as for some rat and chicken liver cells in a reduced serum environment.

This F-12K (Kaighn's) Medium is manufactured as follows:
WithWithout
• L-glutamine• HEPES
• Phenol Red

The complete formulation is available.

Using Ham's F-12K
Ham's F-12K (Kaighn's) Medium contains many components not found in traditional basal media, such as putrescine, thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These additions allow the medium to be supplemented with very low levels of serum or defined components, for some cell types. Ham's F-12K (Kaighn's) Medium contains no proteins or growth factors, and is therefore often supplemented with growth factors and Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell line. Ham's F-12K (Kaighn's) Medium uses a sodium bicarbonate buffer system (2.5 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Dual-site cGMP manufacturing
For supply chain continuity, we manufacture Ham's F-12K (Kaighn's) Medium at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Shipping Condition
Room Temperature
Volume (Metric)
500 ml
Product Line
Gibco™
Form
Liquid
Glutamine
L-Glutamine
Phenol Red Indicator
Phenol Red
Green Features
Sustainable packaging
Quantity
500mL
Glucose
Low Glucose
Sodium Pyruvate Additive
Sodium Pyruvate
HEPES Buffer
No HEPES

Contents & Storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient

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Citations & References