Medio F-12K (de Kaighn) de Ham
Medio F-12K (de Kaighn) de Ham
Citas y referencias (5)
Gibco™

Medio F-12K (de Kaighn) de Ham

El medio F-12K de Ham (Kaighn) es una modificación de la mezcla de nutrientes F12 de Ham. El medio F-12KMás información
Have Questions?
Cambiar vistabuttonViewtableView
Número de catálogoCantidad
21127022500 mL
2112703010 x 500 mL
Número de catálogo 21127022
Precio (MXN)
-
Cantidad:
500 mL
Customize this product
El medio F-12K de Ham (Kaighn) es una modificación de la mezcla de nutrientes F12 de Ham. El medio F-12K de Ham (de Kaighn) se desarrolló para hepatocitos humanos primarios, así como para algunas células hepáticas de pollo y rata en un medio reducido en suero.

Este medio F-12K (de Kaighn) se modifica de la siguiente manera:
ConSin
• L-glutamina• Ácido 4-(2-hidroxietil)piperazin-1-iletanosulfónico (HEPES)
• Rojo de fenol

Está disponible la formulación completa.

Uso de F-12K de Ham
El medio F-12K de Ham (de Kaighn) contiene muchos componentes que no se encuentran en medios basales tradicionales, como putrescina, timidina, hipoxantina y zinc, así como niveles más altos de todos los aminoácidos y piruvato sódico. Estas adiciones permiten que el medio se suplemente con niveles muy bajos de suero o componentes definidos para algunos tipos de células. El medio F-12K de Ham (Kaighn) no contiene proteínas ni factores de crecimiento. Por tanto, con frecuencia se suplementa con factores de crecimiento y suero fetal bovino (SFB). La concentración de SFB se debe optimizar para cada línea celular. El medio F-12K de Ham (de Kaighn) utiliza un sistema de tampón de bicarbonato sódico (2,5 g/l) y, por lo tanto, requiere un ambiente con un 5–10 % de CO2 para mantener el pH fisiológico.

Sistema de elaboración y calidad conforme con las buenas prácticas de fabricación actuales
Para mantener la continuidad de la cadena de suministro, fabricamos el medio F-12K de Ham (de Kaighn) en dos plantas distintas, una ubicada en Grand Island, Nueva York (EE. UU.), y la otra en Escocia, Reino Unido. Ambos sitios cumplen los requisitos de producción según las buenas prácticas de fabricación actuales, cuentan con la certificación ISO 13485 y están registrados en la FDA como fabricantes de dispositivos médicos.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Tipo de célulaHepatocitos humanos
Concentración1 X
Calidad de fabricacióncGMP-compliant under the ISO 13485 standard
Línea de productosGibco
Tipo de productoMedio F-12K (de Kaighn) de Ham
Cantidad500 mL
Duración de almacenamiento12 meses a partir de la fecha de fabricación
Condiciones de envíoTemperatura ambiente
ClasificaciónLibre de material de origen animal
FormularioLíquido
EsterilidadEstéril con filtro
Sterilization MethodEstéril con filtro
Con aditivosBajo nivel de glucosa, Glutamina, Rojo de fenol, Piruvato sódico
Sin aditivosSin HEPES
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 2 a 8 °C. Proteger de la luz
Condiciones de envío: Ambiente

Preguntas frecuentes

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I see a decrease in growth of my culture. What should I do?

Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My cells are not adhering to the culture vessel. What should I do?

This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Medio F-12K (de Kaighn) de Ham FAQs

Citations & References (5)

Citations & References
Abstract
Human MutY homolog, a DNA glycosylase involved in base excision repair, physically and functionally interacts with mismatch repair proteins human MutS homolog 2/human MutS homolog 6.
Authors: Gu Yesong; Parker Antony; Wilson Teresa M; Bai Haibo; Chang Dau-Yin; Lu A-Lien;
Journal:J Biol Chem
PubMed ID:11801590
Adenines mismatched with guanines or 7,8-dihydro-8-oxo-deoxyguanines that arise through DNA replication errors can be repaired by either base excision repair or mismatch repair. The human MutY homolog (hMYH), a DNA glycosylase, removes adenines from these mismatches. Human MutS homologs, hMSH2/hMSH6 (hMutSalpha), bind to the mismatches and initiate the repair on ... More
Stimulation of neuronal neurite outgrowth using functionalized carbon nanotubes.
Authors:Matsumoto K, Sato C, Naka Y, Whitby R, Shimizu N
Journal:Nanotechnology
PubMed ID:20173239
Low concentrations (0.11-1.7 microg ml(-1)) of functionalized carbon nanotubes (CNTs), which are multi-walled CNTs modified by amino groups, when added with nerve growth factor (NGF), promoted outgrowth of neuronal neurites in dorsal root ganglion (DRG) neurons and rat pheochromocytoma cell line PC12h cells in culture media. The quantity of active ... More
Dystrophin deficiency markedly increases enterovirus-induced cardiomyopathy: a genetic predisposition to viral heart disease.
Authors: Xiong Dingding; Lee Gil-Hwan; Badorff Cornel; Dorner Andrea; Lee Sang; Wolf Paul; Knowlton Kirk U;
Journal:Nat Med
PubMed ID:12118246
Both enteroviral infection of the heart and mutations in the dystrophin gene can cause cardiomyopathy. Little is known, however, about the interaction between genetic and acquired forms of cardiomyopathy. We previously demonstrated that the enteroviral protease 2A cleaves dystrophin; therefore, we hypothesized that dystrophin deficiency would predispose to enterovirus-induced cardiomyopathy. ... More
The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain.
Authors: Kuroda Taruho S; Fukuda Mitsunori; Ariga Hiroyoshi; Mikoshiba Katsuhiko;
Journal:J Biol Chem
PubMed ID:11773082
rab27A, which encodes a small GTP-binding protein, was recently identified as a gene in which mutations caused human hemophagocytic syndrome (Griscelli syndrome) and ashen mice, which exhibit defects in melanosome transport as well as in regulated granule exocytosis in cytotoxic T lymphocytes. However, little is known about the molecular mechanism ... More
Role for 18:1 lysophosphatidic acid as an autocrine mediator in prostate cancer cells.
Authors:Xie Y, Gibbs TC, Mukhin YV, Meier KE.
Journal:J Biol Chem
PubMed ID:12084719
Lysophosphatidic acid (LPA) is a lipid mediator that may play an important role in growth and survival of carcinomas. In this study, LPA production and response were characterized in two human prostate cancer (CaP) cell lines: PC-3 and Du145. Bombesin, a neuroendocrine peptide that is mitogenic for CaP cells, stimulated ... More