Medium 199 (2X) This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 21157029

Gibco™  Related applications: Mammalian Cell Culture

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Description

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Medium 199 is modified as follows:

WithWithout
L-glutamineHEPES
Phenol Red
The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2. This Medium 199 is made with Earle's salts.

Product Use
For in vitro diagnostic use.

cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Indicator Type: Calcium Indicator
Indicator Properties: Fluorescent Dye-Based
For Use With (Equipment): Microphotometer, Fluorescence Microscope, Flow Cytometer
Cell Permeability: Cell-Permeant
Product Size: 1 mg
Detection Method: Fluorescent
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 2 – 8°C. Protect from light.
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Documents

Manuals & protocols

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