Thermo Scientific EZ-Link PFP-biotin is a pentafluorophenyl-ester biotinylation reagent that has similar properties to NHS-biotin but is more reactive and will target both primary and secondary amines for biotinylation.
Features of EZ-Link PFP-Biotin:
• Molecular labeling
—biotinylate proteins and nucleic acids for avidin-biotin methods
—can be used to label inside cells (intracellular)
—reacts with primary and secondary amines of proteins and other molecules (unlike typical NHS reagents, which react with primary amines only)
—forms permanent amide bonds; spacer arm cannot be cleaved
—must be dissolved in DMSO or DMF before further dilution in aqueous buffers
• Very short
—spacer arm (total length added to target) is 9.6 angstroms; it consists of the native biotin valeric acid group only
PFP-Biotin is an amine-reactive biotinylation reagent based on activation as as a pentafluorophenyl ester (PFP ester) rather than the usual N-hydroxysuccinimide (NHS) ester. The compound has properties similar to those of NHS-Biotin (Part No. 20217)
but exhibits greater reactivity toward amino groups. Unlike NHS-Biotin, PFP-Biotin will react with both secondary amines and primary amines, enabling it to be used to label nucleic acids as well as proteins. The reaction occurs spontaneously at pH 7 to 9.
We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
For Research Use Only. Not for use in diagnostic procedures.