EZ-Link™ PFP-Biotin

Catalog number: 21218

Thermo Scientific™  Related applications: Protein Labeling & Crosslinking

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


Thermo Scientific EZ-Link PFP-biotin is a pentafluorophenyl-ester biotinylation reagent that has similar properties to NHS-biotin but is more reactive and will target both primary and secondary amines for biotinylation.

Features of EZ-Link PFP-Biotin:

Molecular labeling—biotinylate proteins and nucleic acids for avidin-biotin methods
Membrane-permeable—can be used to label inside cells (intracellular)
Amine-reactive—reacts with primary and secondary amines of proteins and other molecules (unlike typical NHS reagents, which react with primary amines only)
Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
Solubility—must be dissolved in DMSO or DMF before further dilution in aqueous buffers
Very short—spacer arm (total length added to target) is 9.6 angstroms; it consists of the native biotin valeric acid group only

PFP-Biotin is an amine-reactive biotinylation reagent based on activation as as a pentafluorophenyl ester (PFP ester) rather than the usual N-hydroxysuccinimide (NHS) ester. The compound has properties similar to those of NHS-Biotin (Part No. 20217) but exhibits greater reactivity toward amino groups. Unlike NHS-Biotin, PFP-Biotin will react with both secondary amines and primary amines, enabling it to be used to label nucleic acids as well as proteins. The reaction occurs spontaneously at pH 7 to 9.

We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
For Research Use Only. Not for use in diagnostic procedures.


Spacer: Short
Cell Permeability: Cell-Permeant
Label or Dye: Biotin
Chemical Reactivity: Amine
Solubility: DMSO (Dimethylsulfoxide), DMF (Dimethylformamide)
Product Size: 50 mg

Contents & storage

Store desiccated at 4°C. Shipped at ambient temperature.


Manuals & protocols