Probing conformational dynamics of an enzymatic active site by an in situ single fluorogenic probe under piconewton force manipulation.
AuthorsPal N, Wu M, Lu HP
JournalProc Natl Acad Sci U S A
PubMed ID27940917
'Unraveling the conformational details of an enzyme during the essential steps of a catalytic reaction (i.e., enzyme-substrate interaction, enzyme-substrate active complex formation, nascent product formation, and product release) is challenging due to the transient nature of intermediate conformational states, conformational fluctuations, and the associated complex dynamics. Here we report our ... More
Heparin Differentially Impacts Gene Expression of Stromal Cells from Various Tissues.
AuthorsLaner-Plamberger S, Oeller M, Poupardin R, Krisch L, Hochmann S, Kalathur R, Pachler K, Kreutzer C, Erdmann G, Rohde E, Strunk D, Schallmoser K
JournalSci Rep
PubMed ID31076619
Pooled human platelet lysate (pHPL) is increasingly used as replacement of animal serum for manufacturing of stromal cell therapeutics. Porcine heparin is commonly applied to avoid clotting of pHPL-supplemented medium but the influence of heparin on cell behavior is still unclear. Aim of this study was to investigate cellular uptake ... More
Heterogeneous pathway activation and drug response modelled in colorectal-tumor-derived 3D cultures.
AuthorsSchumacher D, Andrieux G, Boehnke K, Keil M, Silvestri A, Silvestrov M, Keilholz U, Haybaeck J, Erdmann G, Sachse C, Templin M, Hoffmann J, Boerries M, Schäfer R, Regenbrecht CRA
JournalPLoS Genet
PubMed ID30925167
Organoid cultures derived from colorectal cancer (CRC) samples are increasingly used as preclinical models for studying tumor biology and the effects of targeted therapies under conditions capturing in vitro the genetic make-up of heterogeneous and even individual neoplasms. While 3D cultures are initiated from surgical specimens comprising multiple cell populations, ... More