Thermo Scientific™

ANB-NOS (N-5-azido-2-nitrobenzoyloxysuccinimide)

Catalog number:  21451

 Related applications: Protein Labeling & Crosslinking

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Thermo Scientific Pierce ANB-NOS is a short-arm (7.7 angstrom) crosslinker that contains an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable nitrophenyl azide.

We no longer recommend the use of this product. A new crosslinker, LC-SDA (NHS-Diazirine), has similar reactivity but incorporates a higher efficiency photoreactive group.


Reactive groups: NHS ester and nitrophenyl azide
Reactive towards: amino groups and any nucleophile
• Nitro group on the phenyl azide ring shifts wavelength for photoactivation to 320-350nm, which is less damaging to proteins and nucleic acids

Product References
Crosslinker Application Guide—search for recent literature references for this product

Related Products
ANB-NOS (N-5-azido-2-nitrobenzoyloxysuccinimide)
For Research Use Only. Not for use in diagnostic procedures.


Crosslinker Type: Heterobifunctional
Labeling Method: Chemical Labeling
Product Line: Pierce™
Product Size: 50 mg
Solubility: DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
Spacer: Short

Contents & storage

Store at -20°C protected from light and moisture.


Manuals & protocols

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