This product is manufactured in our Paisley, Scotland facility and is intended for sale in Europe, Africa, and the Middle East—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Grand Island, US, is recommended for our customers in North America, Latin America, and the Asia/Pacific region. Click to view.
Gibco™
RPMI 1640 Medium
Description
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool. This RPMI is modified as follows:
| With | Without | ||
| • L-glutamine | • HEPES | ||
| • Phenol Red |
The complete formulation is available.
Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g ⁄ L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Product use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco® RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).
cGMP manufacturing and quality system
RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical RPMI 1640 product made in our Grand Island facility (11875-093). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.
Specifications
| Classification: | Animal Origin-Free |
|---|---|
| Culture Environment: | CO2 |
| Green Features: | Sustainable packaging |
| HEPES Buffer: | No HEPES |
| Osmolality: | 260 - 310 mOsm/kg |
| Product Line: | Gibco® |
| Serum Supplementation: | Standard Serum Supplementation |
| Shipping Condition: | Room Temperature |
| Sodium Bicarbonate Buffer: | Sodium Bicarbonate |
| Sodium Pyruvate Additive: | No Sodium Pyruvate |
| Supplementation Required: | Serum |
| Volume (Metric): | 500 ml |
| Form: | Liquid |
| Glutamine: | L-Glutamine |
| Phenol Red Indicator: | Phenol Red |
| pH Range: | 7.0 - 7.4 |
| Concentration: | 1 X |
Contents & storage
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
