Thermo Scientific Pierce SBAP is a short (6.2 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and bromoacetyl reactive groups.
Features of SBAP:
• Reactive groups:NHS ester and bromoacetyl • Reactive toward: amino and sulfhydryl groups • NHS ester reacts with primary amines at pH 7-9 to form a stable amide bond • Bromacetyl group reacts with sulfhydryl groups at pH > 7.5 to form stable thioether bonds • Non-cleavable • Water-insoluble (dissolve first in DMF or DMSO) • Spacer maintains peptide-like character in the crosslinked species • Resulting crosslink is susceptible to acid hydrolysis • Useful for preparing cyclic peptides and peptide conjugates
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
For Research Use Only. Not for use in diagnostic procedures.