RPMI 1640 Medium, HEPES
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RPMI 1640 Medium, HEPES
Gibco™

RPMI 1640 Medium, HEPES

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park MemorialRead more
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Catalog NumberQuantity
22400089500 mL
22400097100 mL
2240010510 x 500 mL
224000711000 mL
224001216 x 1000 mL
Catalog number 22400089
Price (USD)/ 500 mL
42.65
Online exclusive
47.84 
Save 5.19 (11%)
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Quantity:
500 mL
Customize this product
Price (USD)/ 500 mL
42.65
Online exclusive
47.84 
Save 5.19 (11%)
Add to cart
RPMI 1640 Medium, HEPES
Catalog number22400089
Price (USD)/ 500 mL
42.65
Online exclusive
47.84 
Save 5.19 (11%)
-
Add to cart

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:
With
• L-glutamine
• HEPES
• Phenol Red


The complete formulation is available.

Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell TypeLeukemic Cells
ClassificationAnimal Origin-free
Concentration1 X
FormLiquid
Product TypeRPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Serum LevelStandard Serum Supplementation
Shelf Life12 Months From Date of Manufacture
SterilitySterile-filtered
With AdditivesGlutamine, HEPES, Phenol Red
Without AdditivesNo Sodium Pyruvate
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity500 mL
Shipping ConditionRoom Temperature
Unit Size500 mL
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture