Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications.
This Opti-MEM® is modified as follows:
• Phenol Red
The complete formulation is confidential. For more information, please contact Technical Services.
Gibco® Opti-MEM® is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%.
Product Intended Use For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System Gibco® Opti-MEM® is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® Opti-MEM® product made in our Scotland facility (22600-134). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Gibco® Opti-MEM® uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment and filtration at the time of preparation (see protocol for details).
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Phenol Red Indicator:
10 x 1 L
Contents & storage
Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 30 months from date of manufacture