The Thermo Scientific Power Stainer (Cat. No. 22833) consists of a Thermo Scientific Power Station (Cat. No. 22838) with activated Staining Software and a Thermo Scientific Power Stain Cassette (Cat. No. 22836). It is designed for rapid Coomassie staining of proteins in polyacrylamide gels and subsequent removal of stain from the gel background. Traditional Coomassie staining techniques require one hour to overnight of staining and destaining to achieve the desired results. When used in conjunction with Thermo Scientific Midi and Mini Gel Power Staining Kits, the Thermo Scientific Power Stainer is designed to provide staining efficiency in as few as 6 minutes that is equivalent to, or better than, traditional Coomassie staining techniques. This significant reduction in protein staining time is accomplished by fixing the protein to the gel and electrophoretically transporting the negatively charge Coomassie R250 dye rapidly through the gel matrix. The dye passes through the polyacrylamide and ionically binds to the protein, resulting in crisp blue bands with minimal background. The system has been verified to work with commonly used pre-cast and homemade SDS-PAGE gels.
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