Thermo Scientific™

Pierce™ Coomassie (Bradford) Protein Assay Kit

Catalog number:  23200

Related applications:

Protein Assays and Analysis

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Description

The Pierce Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent for measurement of total protein concentration compared to a protein standard. The kit includes Coomassie Protein Assay Reagent and a package of Albumin Standard ampules.

Compare all available Bradford assays ›

The Pierce Coomassie Protein Assay Kit is a ready-to-use formulation of the popular assay reagent originally described by Bradford in 1976. When mixed with a protein solution, the acidic Coomassie-dye reagent changes color from brown to blue in proportion to the amount of protein present in the sample. Protein determinations are made by comparison to the color response of protein assay standards, usually prepared as a series of known dilutions of bovine serum albumin (BSA) or bovine gamma globulin (BGG). The simple procedure is adaptable to nearly any volume scale, including test tubes, cuvettes, and microplates. The protein assay is compatible with most salts, solvents, buffers, thiols, reducing substances, and metal chelating agents encountered in protein samples.

Features of the Coomassie Protein Assay Kit include:
Bradford reagent—stable, ready-to-use kit of the classical Bradford assay reagent
Colorimetric—measure with a standard spectrophotometer or plate reader at 595 nm
Easy to use—single reagent; no working reagent preparation required
Fast—almost immediate color development; add, mix, and read results
Assay range—detects protein concentration in the range 1 to 1500 µg/mL
Flexible—microplate and cuvette protocols provided and adaptable to several target working ranges

How the Coomassie (Bradford) Assay detects protein
Development of color in Coomassie dye-based (Bradford) protein assays has been associated with the presence of certain basic amino acids (primarily arginine, lysine, and histidine) in the protein. Van der Waals forces and hydrophobic interactions also participate in the binding of the dye by protein. The number of Coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Free amino acids, peptides, and low molecular weight proteins do not produce color with Coomassie dye reagents. In general, the mass of a peptide or protein must be at least 3,000 daltons to be assayed with this reagent.

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Pierce Coomassie Plus (Bradford) Assay Kit
Pierce Detergent Compatible Bradford Assay Kit
Ampule Breakers
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application): Solution-based Detection, Absorbance
Specificity: Not Target-Specific
Assay: Protein Quantitation Assay
Product Line: Pierce™
Detection Method: Colorimetric
For Use With (Equipment): Spectrophotometer, Microplate Reader

Contents & storage

Sufficient For:
  • 630 tube assays or 3800 microplate assays
  • Pierce Coomassie Assay Reagent, 950 mL
  • Albumin Standard Ampules, 2 mg/mL, 10 x 1 mL
Store Coomassie Protein Assay Reagent at 4°C. Store unopened Albumin Standard Ampules at room temperature.