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Thermo Scientific™
Pierce™ Fluorescent Protease Assay Kit
The Thermo Scientific Pierce Fluorescent Protease Assay Kit measures total protease activity in samples, providing a means to assess theRead more
| Catalog Number | Quantity |
|---|---|
| 23266 | 2000 Test Kit |
Catalog number 23266
Price (MXN)
-
Quantity:
2000 Test Kit
The Thermo Scientific Pierce Fluorescent Protease Assay Kit measures total protease activity in samples, providing a means to assess the progress of protease isolation procedures or quantify protease contamination in biological samples.Casein substrate—measure activity of any protease that cleaves casein into peptide fragments Trypsin standard—quantify protease activity relative to trypsin, a universally accepted reference Sensitive—1000 times more sensitive than assays that use unmodified forms of casein Simple and fast—test tube and microplate protocols are completed in less than one hour Homogenous—addition steps only; no separation, transfer or stop steps required Customizable—easily adapt time, temperature and pH to optimize sensitivity for proteases of interest Assess functional integrity and overall progress of protease purification Quantify protease contamination in protein samples Characterize the activity of unusual proteases compared to trypsin Evaluate buffer conditions for their affects on protease function and activity Substrate: fluorescein-labeled casein (FITC-casein); available separately as Part No. 23267 Basis of assay: change in fluorescence resonance (FRET) or fluorescence polarization (FP) Measurement: instrument with fluorescein excitation and emission filters (485/538nm)
This kit includes fluorescein-labeled casein as a substrate for assessing protease activity in a sample by either fluorescence resonance energy transfer (FRET) with a standard fluorometer or fluorescence polarization (FP) with capable instrumentation. FTC-Casein is native casein that has been labeled using a large molar excess of fluorescein isothiocyanate (FITC). Fluorescence properties of this heavily-labeled, intact protein substrate change dramatically upon digestion by proteases, resulting in a measurable indication of proteolysis.
Features of the Fluorescent Protease Assay Kit:
Applications:
Additional Specifications:
Related Products
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AssayProtease Assay
For Use With (Application)Fluorescence Polarization, FRET
For Use With (Equipment)Microplate Reader
Product LinePierce
Product TypeProtein Activity Assay
Quantity2000 Test Kit
SubstrateCasein
Substrate PropertiesProtein-Based Substrate
Substrate TypeProtease Substrate
Sufficient For2000 Microplate Assays
TargetProteases
Detection MethodFluorescence
Immunoassay Kit Format96-well Plate
Label TypeClassic Dye
Unit SizeEach
Contents & Storage
Sufficient For: 2000 microplate assays using an instrument fitted with a fluorescein filter set (ex/em 485 nm/538 nm)
• FTC-Casein, lyophilized, 2.5 mg
• TPCK Trypsin Standard, 50 mg
• TBS Buffer Pack (makes 500 mL), 1 pack
Store FTC-Casein and TPCK Trypsin at 4°C.
• FTC-Casein, lyophilized, 2.5 mg
• TPCK Trypsin Standard, 50 mg
• TBS Buffer Pack (makes 500 mL), 1 pack
Store FTC-Casein and TPCK Trypsin at 4°C.