Thermo Scientific™

Pierce™ Quantitative Peroxide Assay Kit (Aqueous)

Catalog number:  23280

 Related applications:

Protein Assays and Analysis

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The Thermo Scientific Pierce Quantitative Peroxide Assay Kit (aqueous-compatible formulation) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange (XO) reagent.

This aqueous-compatible peroxide assay kit detects peroxides based on oxidation of ferrous to ferric ion in the presence of xylenol orange. The kit's formulation includes sorbitol, which provides sensitivity enhancement. Most proteins do not interfere with this assay, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects. This kit provides for maximum sensitivity with non-lipid samples.

Features of the Quantitative Peroxide Assay Kit:

Peroxides—detects and measures hydrogen peroxide (H2O2) levels in biological and other liquids samples
Convenient—no proprietary reagents involved but the ready-to-use kit removes the variability, difficulty and added expense of formulating the reagents yourself

• General hydrogen peroxide (H2O2) measurement in protein samples
• Quantitation of peroxides in detergents
• Assessment of the effects of peroxides on cellular activity
• Determination of protein glycation; Measurement of peroxide accumulation in lipids

In this assay, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With this aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm. Peroxide levels in test samples can be determined by calculation from the known extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.

Related Products
Pierce™ Quantitative Peroxide Assay Kit (Lipid)
Pierce™ Peroxide Assay Reagent A
Pierce™ Peroxide Assay Reagent B
Pierce™ Peroxide Assay Reagent C
For Research Use Only. Not for use in diagnostic procedures.


Bulk or Custom Form: Bulk Custom Form 1
Target Specificity: Not Target-Specific
Assay Type: [other]
Detection Method: Colorimetric
For Use With (Equipment): Microplate Reader, Spectrophotometer
Product Line: Pierce™
Product Size: 100 mL
Technique: Absorbance, Solution-based Detection

Contents & storage

Sufficient For: 100 tube assays or 500 microplate assays with non-lipid samples
• Reagent A, 1 mL
• Reagent B, 2 x 50 mL

Store at 4°C.


Manuals & protocols