O-GlcNAc Western Blot Detection Kit

Catalog number:  24565

Thermo Scientific™  Related applications: Protein Assays and Analysis

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The Thermo Scientific Pierce O-GlcNAc Western Blot Detection Kit contains a specific antibody for the O-GlcNAc post-translational modification (PTM), as well as detection reagents needed to perform a complete detection experiment.

Features of the O-GlcNAc Western Blot Detection Kit:

• Highly specific monoclonal antibody against O-GlcNAc modification
• Speed and sensitivity of chemiluminescent detection
• Complete kit contains cell lysis and detection reagents

The Pierce O-GlcNAc Western Blot Detection Kit contains a monoclonal antibody which has specific affinity for the beta-O-linked serine or threonine GlcNAc modification. There is no cross-reactivity with the alpha-O-GlcNAc linkage, the α/β-O-GalNAc modification or other N-linked oligosaccharides. The kit also includes cell lysis reagent, secondary antibody and chemiluminescent substrate needed to perform a complete detection experiment.

Product Details:
Glycosylation and phosphorylation are two of the most extensively studied post-translational modifications because of their important role in the signaling pathways that regulate cellular response. The O-glycosidically linked modifications of serine or threonine residues are found on proteins from essentially all eukaryotic cells. Proteins in both the cytoplasm and nucleus can be found with this specific posttranslational modification. Although not precisely known, the function of the β-O-linked N-acetylglucosamine (O-GlcNAc) modification may serve as an alternative to phosphorylation, as a modulator of protein interactions in multimeric complexes or as a fortification against proteolytic attack.

The O-GlcNAc modification is seemingly related to phosphorylation in many respects. Notably, the modification is controlled similarly to that of phosphorylation. All O-GlcNAc-modified proteins currently known are also phosphoproteins. In addition, there is an apparent reciprocal relationship between these two important posttranslational modifications within a single protein. Therefore, there is considerable interest in the O-GlcNAc modification.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Western Blot
Product Line: Pierce™
Product Size: 255 mL
Reactivity: All species
Substrate: Luminol

Contents & storage

• M-PER™ Mammalian Protein Extraction Reagent, 25 mL, store at room temperature
• Dilution Buffer (also functions as Blocking Buffer) (1X), 2 × 5 mL, store at 4°C
• BupH™ Phosphate Buffered Saline, 17 packs, store at room temperature
• Surfact-Amps™ 2 (1% Tween™-2 Detergent), 3 × 1 mL, store at room temperature
• Goat Anti-Mouse IgM (µ), HRP Conjugate, 75 µg, store at 4°C
• SuperSignal™ West Dura Luminol/Enhancer Solution, 5 mL, store at room temperature
• SuperSignal West Dura Stable Peroxide Solution, 5 mL, store at room temperature


Manuals & protocols

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