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Gibco™

Trypsin-EDTA (0.05%), phenol red

Green features
Catalog number: 25300054
Gibco™

Trypsin-EDTA (0.05%), phenol red

Green features
Catalog number: 25300054
Catalog Number
25300054
also known as 25300-054
Unit Size
100 mL
Price (USD)
14.39
Your Price:
Availability
***
Quantity
Catalog Number
25300062
also known as 25300-062
Unit Size
500 mL
Price (USD)
44.64
Your Price:
Availability
***
Quantity
Catalog Number
25300120
also known as 25300-120
Unit Size
20 x 100 mL
Price (USD)
250.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
25300054
also known as 25300-054
100 mL
14.39
Your Price:
***
25300062
also known as 25300-062
500 mL
44.64
Your Price:
***
25300120
also known as 25300-120
20 x 100 mL
250.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Gibco® Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco® trypsin solutions and recommended dissociation conditions.

We offer a variety of trypsin formulations and animal origin-free TrypLE™ reagent that feature:

Quality Testing
Documented Traceability
Dual-site cGMP Manufacturing

This trypsin solution is modified as follows:

With
• EDTA
• Phenol Red

The complete formulation is available.

Quality Testing
Gibco® Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, prior to manufacturing, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Documented Traceability
We can provide detailed documentation to meet your regulatory needs. Gibco® Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP Manufacturing and Quality System
Gibco® Trypsin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® Trypsin solution made in our Scotland facility (25300-096). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.

Specifications

Source
Animal Origin
Classification
Animal Origin
pH
7.1 to 8
Concentration
1 X
Product Type
Cell Culture Dissociation Reagents
Sterility
Sterile
Chelators
EDTA
pH Range
7.2 - 8.0
Osmolality
270 - 310 mOsm/kg
Shipping Condition
Wet ice
Tested For
In Vitro Bioassay
Product Line
Gibco™
Form
Liquid
Phenol Red Indicator
Phenol Red
Shelf Life
24 Months
Green Features
Sustainable packaging
Quantity
100mL
Reagent Type
Trypsin
Cell Type
Mammalian
Solution Type
Trypsin

Contents & Storage

Storage conditions: -5°C to -20°C
Shelf life: 24 months from date of manufacture

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Frequently asked questions (FAQs)

Citations & References