PageRuler™ vorgefärbte Proteinleiter, 10 bis 180 kDa
PageRuler™ vorgefärbte Proteinleiter, 10 bis 180 kDa
Thermo Scientific™

PageRuler™ vorgefärbte Proteinleiter, 10 bis 180 kDa

Die Thermo Scientific PageRuler Vorgefärbte Proteinleiter ist eine Mischung aus zehn blau-, orange- und grüngefärbten Proteinen (10 bis 180 kDa)Weitere Informationen
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KatalognummerMenge
266162 x 250 μl
26616X48 x 250 μL
2661710 x 250 μl
Katalognummer 26616
Preis (EUR)
157,65
Exklusiv online
186,00
Ersparnis 28,35 (15%)
Each
Zum Warenkorb hinzufügen
Menge:
2 x 250 μl
Großbestellung oder individuelle Größe anfordern
Preis (EUR)
157,65
Exklusiv online
186,00
Ersparnis 28,35 (15%)
Each
Zum Warenkorb hinzufügen
Die Thermo Scientific PageRuler Vorgefärbte Proteinleiter ist eine Mischung aus zehn blau-, orange- und grüngefärbten Proteinen (10 bis 180 kDa) zur Verwendung als Größenstandard in der Protein-Elektrophorese (SDS-PAGE) und für das Western Blot-Verfahren. Die Proteinleiter wird in einem gebrauchsfertigen Format geliefert zum direkten Auftragen auf Gele. Vor der Verwendung muss kein Probenpuffer erwärmt, reduziert oder hinzugefügt werden.

Alle anderen Proteinstandards und -leitern anzeigen und vergleichen ›

Anwendungen
• Überwachen der Proteinmigration während der SDS-Polyacrylamid-Gelelektrophorese
• Überwachen der Proteinübertragung auf Membranen nach Western Blotting
• Größenbestimmung von Proteinen in SDS-Polyacrylamid-Gelen und Western Blots
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
NachweisverfahrenKolorimetrisch, NIR-Fluoreszenz (700 nm), RGB-Fluoreszenz (555 nm)
GelkompatibilitätBolt™ Bis-Tris Plus Gele, Novex™ Tricin-Gele, Novex™ Tris-Glycin-Gele, NuPAGE™ Bis-Tris-Gele, NuPAGE™ Tris-Acetat-Gele, SDS-PAGE-Gele
Molekulargewicht180, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa
Menge2 x 250 μl
Sofort einsatzbereitJa
VersandbedingungZugelassen für den Versand auf Nass- oder Trockeneis
Number of Markers10
ProduktliniePageRuler
ProdukttypProteinleiter
Größenbereich10 bis 180 kDa
Stain Type3 Farben: Blau, orange, grün
System TypeWestern Blotting, SDS-PAGE
Unit SizeEach
Inhalt und Lagerung
Inhalt: zwei 250 μL-Fläschchen

Lagerpuffer: 62,5 mmol Tris-H3PO4 (pH 7,5 bei 25 °C), 1 mmol EDTA, 2 % SDS, 10 mmol DTT, 1 mmol NaN3 und 33 % Glycerin

Lagerung: Nach Erhalt bei -20 °C lagern

Häufig gestellte Fragen (FAQ)

Why do Thermo Scientific prestained protein ladders not show the real protein sizes?

Coupling of chromophores to proteins affects the apparent molecular weight of proteins in SDS-PAGE relative to unstained standards. The apparent molecular weight of prestained protein standards is calibrated in the classical TRIS glycine-SDS Laemmli system, however prestained proteins may have different mobility in other electrophoresis buffer and gel systems. It should also be noted that the sizing of proteins by gel electrophoresis does not give an exact value and depends on the protein sequence and post-modification.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The upper bands of the ladder are missing. What could be the reason?

The upper bands of the ladder may be degraded by proteases. Ladder, gel, buffer, pipettes, pipette tips, or equipment can be contaminated by proteases during usage. A general recommendation would be to avoid working with proteases in the same room. We would recommend preparing fresh solutions, cleaning the equipment, and using clean pipettes and tips. If the ladder itself is contaminated, please use a new tube of the ladder.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do the proteins in Thermo Scientific protein ladders have a His-Tag or would otherwise react with an anti-His-Tag antibody?

No, proteins in Thermo Scientific protein ladders are not His tagged. However, non-specific interaction between the ladder proteins and primary or secondary antibodies is possible and some His-Tag detection systems, such as Thermo Scientific 6xHis Protein Tag Stain Reagent Kit, show non-specific interaction. The protein ladder bands are more readily detected when using high antibody concentrations. The non-specific cross-reactivity is difficult to predict, it often has a different pattern dependent on the antibodies used in each individual experiment. The most general way to handle this problem would be to use lower concentrations of antibodies and to use lower amount of protein ladders. It may also be useful to leave one empty well between the ladder and the sample to overcome a possible leakage of the signal to the nearby sample lane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can Thermo Scientific protein ladders be detected by Strep-Tactin conjugates?

PageRuler Unstained protein ladders can be detected directly on Western blots by using Strep-Tactin conjugates or an antibody against the Strep-tag II sequence. All PageRuler and Spectra ladder proteins contain an integral Strep-tag II sequence, however the prestained ladders cannot be detected by Strep-Tactin conjugates.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why are the PageRuler and Spectra ladder bands detected with antibodies against eukaryotic proteins?

All PageRuler and Spectra ladder bands are recombinant prokaryotic proteins purified from E. coli cells. None of them are related to eukaryotic proteins, however this cannot exclude the possibility that the ladder proteins may possess an epitope that is cross-reactive with the antibody used.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all PageRuler™ vorgefärbte Proteinleiter, 10 bis 180 kDa FAQs