Thermo Scientific™

Pierce™ Concentrated Buffer Stocks (10X and 20X)

Catalog number: 28360
Thermo Scientific™

Pierce™ Concentrated Buffer Stocks (10X and 20X)

Catalog number: 28360
Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.
 
Catalog Number
28360
Unit Size
500 mL
Concentration
20 X
Product Line
Pierce™, Tween™
Product Type
TBS Tween 20 Buffer
Quantity
500 mL
Price (USD)
Price: 107.00
Your Price:
Availability
***
Full specifications
Quantity
Concentration20 X
FormatLiquid
Product LinePierce™, Tween™
Product TypeTBS Tween 20 Buffer
Quantity500 mL
pH7.5
Unit Size500 mL
Catalog Number
Unit Size
500 mL
Concentration
20 X
Product Line
Pierce™, Tween™
Product Type
PBS Tween 20 Buffer
Quantity
500 mL
Price (USD)
Price: 107.00
Your Price:
Availability
***
Full specifications
Quantity
Concentration20 X
FormatLiquid
Product LinePierce™, Tween™
Product TypePBS Tween 20 Buffer
Quantity500 mL
pH7.5
Unit Size500 mL
Catalog Number
Unit Size
500 mL
Concentration
20 X
Product Line
Pierce™
Product Type
TBS Buffer
Quantity
500 mL
Price (USD)
Price: 106.00
Your Price:
Availability
***
Full specifications
Quantity
Concentration20 X
FormatLiquid
Product LinePierce™
Product TypeTBS Buffer
Quantity500 mL
For Use With (Application)ELISA, Western Blotting
pH7.2
Reagent TypeTBS Buffer
Unit Size500 mL
Catalog Number
Unit Size
500 mL
Concentration
20 X
Product Line
Pierce™
Product Type
Phosphate-Buffered Saline (PBS)
Quantity
500 mL
Price (USD)
Price: 128.00
Your Price:
Availability
***
Full specifications
Quantity
Concentration20 X
FormatLiquid
Product LinePierce™
Product TypePhosphate-Buffered Saline (PBS)
Quantity500 mL
pH7.5
Unit Size500 mL
Catalog Number
Unit Size
1 L
Concentration
10 X
Product Line
Pierce™
Product Type
Tris-Glycine SDS Buffer
Quantity
1 L
Price (USD)
Price: 82.50
Your Price:
Availability
***
Full specifications
Quantity
Concentration10 X
Product LinePierce™
Product TypeTris-Glycine SDS Buffer
Quantity1 L
pH8.3
Reagent TypeRunning Buffer
Unit Size1 L
Catalog Number
Unit Size
500 mL
Concentration
20 X
Product Line
Pierce™
Product Type
Borate Buffer
Quantity
500 mL
Price (USD)
Price: 106.00
Your Price:
Availability
***
Full specifications
Quantity
Concentration20 X
FormatLiquid
Product LinePierce™
Product TypeBorate Buffer
Quantity500 mL
pH8.5
Unit Size500 mL
Catalog Number
Unit Size
500 mL
Concentration
20 X
Product Line
Pierce™
Product Type
Modified Dulbecco's PBS Buffer
Quantity
500 mL
Price (USD)
Price: 116.00
Your Price:
Availability
***
Full specifications
Quantity
Concentration20 X
FormatLiquid
Product LinePierce™
Product TypeModified Dulbecco's PBS Buffer
Quantity500 mL
pH7.4
Unit Size500 mL
Catalog Number
Unit Size
1 L
Concentration
10 X
Product Line
Pierce™
Product Type
Tris-Glycine Buffer
Quantity
1 L
Price (USD)
Price: 82.50
Your Price:
Availability
***
Full specifications
Quantity
ColorClear
Concentration10 X
Product LinePierce™
Product TypeTris-Glycine Buffer
Quantity1 L
For Use With (Application)Tris-Glycine Gel Electrophoresis
pH8.5
Reagent TypeTransfer Buffer
Unit Size1 L
Catalog Number
Unit Size
1 L
Concentration
10X
Product Line
Pierce™
Product Type
10X TBE Buffer
Quantity
1 L
Price (USD)
Price: 139.00
Your Price:
Availability
***
Full specifications
Quantity
Concentration10X
Product LinePierce™
Product Type10X TBE Buffer
Quantity1 L
For Use With (Application)Agarose Gel Electrophoresis, Polyacrylamide Gel Electrophoresis
Reagent TypeRunning Buffer
Unit Size1 L
Catalog Number
Unit Size
500 mL
Concentration
20 X
Product Line
Pierce™
Product Type
Modified Dulbecco's PBS Tween 20 Buffer
Quantity
500 mL
Price (USD)
Price: 117.00
Your Price:
Availability
***
Full specifications
Quantity
Concentration20 X
FormatLiquid
Product LinePierce™
Product TypeModified Dulbecco's PBS Tween 20 Buffer
Quantity500 mL
pH7.4
Unit Size500 mL
Showing 10 of 11Show more
Catalog NumberSpecificationsUnit SizeConcentrationProduct LineProduct TypeQuantityPrice (USD)AvailabilityQuantity
28360Full specifications
500 mL20 XPierce™, Tween™TBS Tween 20 Buffer500 mL
Price: 107.00
Your Price:
***
Concentration20 X
FormatLiquid
Product LinePierce™, Tween™
Product TypeTBS Tween 20 Buffer
Quantity500 mL
pH7.5
Unit Size500 mL
28352Full specifications
500 mL20 XPierce™, Tween™PBS Tween 20 Buffer500 mL
Price: 107.00
Your Price:
***
Concentration20 X
FormatLiquid
Product LinePierce™, Tween™
Product TypePBS Tween 20 Buffer
Quantity500 mL
pH7.5
Unit Size500 mL
28358Full specifications
500 mL20 XPierce™TBS Buffer500 mL
Price: 106.00
Your Price:
***
Concentration20 X
FormatLiquid
Product LinePierce™
Product TypeTBS Buffer
Quantity500 mL
For Use With (Application)ELISA, Western Blotting
pH7.2
Reagent TypeTBS Buffer
Unit Size500 mL
28348Full specifications
500 mL20 XPierce™Phosphate-Buffered Saline (PBS)500 mL
Price: 128.00
Your Price:
***
Concentration20 X
FormatLiquid
Product LinePierce™
Product TypePhosphate-Buffered Saline (PBS)
Quantity500 mL
pH7.5
Unit Size500 mL
28362Full specifications
1 L10 XPierce™Tris-Glycine SDS Buffer1 L
Price: 82.50
Your Price:
***
Concentration10 X
Product LinePierce™
Product TypeTris-Glycine SDS Buffer
Quantity1 L
pH8.3
Reagent TypeRunning Buffer
Unit Size1 L
28341Full specifications
500 mL20 XPierce™Borate Buffer500 mL
Price: 106.00
Your Price:
***
Concentration20 X
FormatLiquid
Product LinePierce™
Product TypeBorate Buffer
Quantity500 mL
pH8.5
Unit Size500 mL
28344Full specifications
500 mL20 XPierce™Modified Dulbecco's PBS Buffer500 mL
Price: 116.00
Your Price:
***
Concentration20 X
FormatLiquid
Product LinePierce™
Product TypeModified Dulbecco's PBS Buffer
Quantity500 mL
pH7.4
Unit Size500 mL
28363Full specifications
1 L10 XPierce™Tris-Glycine Buffer1 L
Price: 82.50
Your Price:
***
ColorClear
Concentration10 X
Product LinePierce™
Product TypeTris-Glycine Buffer
Quantity1 L
For Use With (Application)Tris-Glycine Gel Electrophoresis
pH8.5
Reagent TypeTransfer Buffer
Unit Size1 L
28355Full specifications
1 L10XPierce™10X TBE Buffer1 L
Price: 139.00
Your Price:
***
Concentration10X
Product LinePierce™
Product Type10X TBE Buffer
Quantity1 L
For Use With (Application)Agarose Gel Electrophoresis, Polyacrylamide Gel Electrophoresis
Reagent TypeRunning Buffer
Unit Size1 L
28346Full specifications
500 mL20 XPierce™Modified Dulbecco's PBS Tween 20 Buffer500 mL
Price: 117.00
Your Price:
***
Concentration20 X
FormatLiquid
Product LinePierce™
Product TypeModified Dulbecco's PBS Tween 20 Buffer
Quantity500 mL
pH7.4
Unit Size500 mL
Showing 10 of 11Show more
Benefits of using Pierce Concentrated Buffer stock solutions include:
Convenient—no weighing, no pH adjustment, no need to stock individual components
Improved accuracy—ready-to-use formula, easily diluted without weighing chemical components
Consistent quality—our quality control ensures that every prep will yield the same, consistent buffer

20X PBS Tween-20 can be used to prepare PBS-Tween (PBS-T) wash buffers for ELISA, western, and other immunoassays, as well as blocking buffer for plate-based assays. When diluted to 1X with water, 20X PBS Tween-20 makes 10 mM sodium phosphate, 0.15 M NaCl, 0.05% Tween™ 20 buffer at pH 7.5.

20X TBS Tween-20 (Tris-buffered saline with Tween™ 20 detergent) can be used to prepare TBS-Tween (TBS-T) wash buffers or blocking buffer diluent for applications such as ELISA, western blotting, and other immunoassays. When diluted 20-fold in water, a TBS Tween-20 buffer containing 25 mM Tris, 0.15 M NaCl, 0.05% Tween-20 at pH 7.5 is ready for use.

20X Tris-Buffered Saline (TBS) is a stock solution for preparing Tris-NaCl buffer for use as a wash buffer and antibody diluent for ELISA, western blotting, and other immunoassays. 20X Tris Buffered Saline (TBS) makes 25 mM Tris, 0.15 M NaCl with pH 7.2 to 7.5 when diluted to 1X with water.

20X Phosphate-Buffered Saline (PBS) is ideal for preparing PBS buffers for use in crosslinking, biotinylation, and fluorescent labeling reactions requiring an amine-free buffer. When diluted 20-fold in water, the solution yields 10 mM sodium phosphate, 0.15 M NaCl at pH 7.5.

10X Tris-Glycine-SDS Buffer makes 0.025 M Tris, 0.192M glycine, 0.1% sodium dodecyl sulfate (SDS) at pH 8.5 when diluted to 1X with water.

20X Borate Buffer is ideal for preparing sodium borate buffer solutions for use in protein modification procedures requiring amine-free buffer at an alkaline pH. 20X Borate Buffer makes 50 mM borate at pH 8.5 when diluted to 1X with water.

20X Modified Dulbecco's PBS is used for preparing physiological Na- and K-phosphate buffered saline (D-PBS) used for wash buffers and antibody diluents in immunoassays like ELISA and western blot. 20X Modified Dulbecco's PBS makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, pH 7.4 when diluted to 1X with water.

10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.5) transfer buffer used for western blotting using Tris-glycine gel electrophoresis. When diluted 10-fold in water or 20% methanol, the solution yields 0.025 M Tris, 0.192 M glycine at pH 8.5.

10X TBE Buffer stock solution is ideal for casting and preparing Tris-borate-EDTA (TBE) running buffer used for agarose and polyacrylamide gel electrophoresis of nucleic acids. When diluted to 1X, the TBE Buffer contains 0.089 M Tris, 0.089 M borate, 2 mM EDTA with pH 8.2 to 8.4.

20X Modified Dulbecco's PBS Tween-20 will make physiological sodium- and potassium-phosphate buffered saline (D-PBS) to be used as a wash buffer for ELISA, western, and other immunoassays or as diluent or blocking buffer for plate-based assays. 20X Modified Dulbecco's PBS Tween-20 (D-PBS-T) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, 0.05% Tween™ 20 at pH 7.4 when diluted to 1X with water.

20X TAE Buffer is a space-saving stock solution that is ideal for casting and preparing Tris-acetate EDTA (TAE) running buffer for agarose gel electrophoresis of nucleic acids. When diluted to 1X with water, a 0.04 M Tris, 0.04 M acetate, 1 mM EDTA buffer at pH 8.2 to 8.4 is ready for use.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20–25°C.