Thermo Scientific™

BupH™ Tris-HEPES-SDS Running Buffer

Catalog number:  28398

 Related applications:

Protein Electrophoresis & Western Blotting

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Thermo Scientific BupH Tris-HEPES-SDS Buffer Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of running buffer for gel electrophoresis (SDS-PAGE) with Thermo Scientific Precise Precast Protein Gels.

Features of Thermo Scientific BupH Tris-HEPES-SDS Buffer Packs:

Tris-HEPES-SDS buffer—dissolved in 500 mL of water, each pack makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

• Running buffer for SDS-PAGE with Thermo Scientific Precise Precast Gels (e.g., Part No. 25224)

BupH Dry-Blend Packs of Tris-HEPES-SDS buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8. HEPES is 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid.

Related Products
Pierce™ 20X Tris-HEPES-SDS Buffer
For Research Use Only. Not for use in diagnostic procedures.


Gel Type: Tris-HEPES Gel
Buffer Type: Running Buffers
Concentrated: Dry Blend
Gel Compatibility: Precise™ Tris-HEPES Gels
Product Line: BupH™
Product Size: 10 packs

Contents & storage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.


Manuals & protocols