Thermo Scientific™

Pierce™ ECL Western Blotting Substrate

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Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Pierce ECL Plus Western Blotting Substrate characteristics include:
Sensitivity: low picogram
Stability: 1-hr working solution stability, 1-year kit stability at 4°C
Compatibility: nitrocellulose and PVDF membranes
Signal duration: 1 to 2 hours
Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–10 µg/mL)
Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 µg/mL)

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Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity. It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.

Because the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare.

For Research Use Only. Not for use in diagnostic procedures.


Product Line: Pierce™
Recommended Antibody Concentrations: 2°: 1:1-15K (0.07-1µg/mL), 1°: 1:1K (0.2- 10µg/mL) 
Signal Duration: 1 to 2 hr
Substrate Properties: Chemical Substrate
Detection Method: Chemiluminescent
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Conjugate: HRP
Description: Pierce ECL Western Blotting Substrate
Kit Contents: Luminol/Enhancer, 250mL
Quantity: 500 mL Kit
Sensitivity: Low picogram
Storage Requirements: 2°C to 8°C
Sufficient For: 50 mini-gel blots; 4000cm2 of membrane

Contents & storage

Upon receipt store at 2-8°C. Product is shipped at ambient temperature.