Thermo Scientific™

Pierce™ ECL Western Blotting Substrate

Catalog number: 32106
Thermo Scientific™

Pierce™ ECL Western Blotting Substrate

Catalog number: 32106
Catalog Number
32106
Unit Size
500 mL
Price (USD)
231.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
50 mL
Price (USD)
68.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
250 mL
Price (USD)
172.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
32106500 mL
231.00
Your Price:
***
3210950 mL
68.00
Your Price:
***
32209250 mL
172.00
Your Price:
***
Product Overview
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Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Pierce ECL Plus Western Blotting Substrate characteristics include:
Sensitivity: low picogram
Stability: 1-hr working solution stability, 1-year kit stability at 4°C
Compatibility: nitrocellulose and PVDF membranes
Signal duration: 1 to 2 hours
Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–10 µg/mL)
Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 µg/mL)

Compare and learn more about our western blot chemiluminescent substrates ›
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Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity. It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.

Because the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Recommended Antibody Concentrations
2°: 1:1-15K (0.07-1µg/mL), 1°: 1:1K (0.2- 10µg/mL) 
Signal Duration
1 to 2 hr
Conjugate
HRP
Detection Method
Chemiluminescent
Form
Liquid
Product Line
Pierce™
Quantity
500 mL Kit
Sensitivity
Low picogram
Substrate
Horseradish Peroxidase
Substrate Properties
Chemical Substrate
Substrate Type
HRP (Horseradish Peroxidase) Substrate
Sufficient For
50 mini-gel blots; 4000cm2 of membrane
Description
Pierce ECL Western Blotting Substrate
Kit Contents
Luminol/Enhancer, 250mL
Product Type
Substrate
Storage Requirements
2°C to 8°C

Contents & Storage

Upon receipt store at 2-8°C. Product is shipped at ambient temperature.

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