Thermo Scientific™

Pierce™ ECL Western Blotting Substrate

Catalog number:  32109

Related applications:

Protein Electrophoresis & Western Blotting

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Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Pierce ECL Plus Western Blotting Substrate characteristics include:
Sensitivity: low picogram
Stability: 1-hr working solution stability, 1-year kit stability at 4°C
Compatibility: nitrocellulose and PVDF membranes
Signal duration: 1 to 2 hours
Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–10 µg/mL)
Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 µg/mL)

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Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity. It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.

Because the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare.

For Research Use Only. Not for use in diagnostic procedures.


Recommended Antibody Concentrations: 2°: 1:1-15K (0.07-1µg/mL), 1°: 1:1K (0.2- 10µg/mL)
Signal Duration: 1 to 2 hr
Detection Method: Chemiluminescent
Form: Liquid
Product Line: Pierce™
Quantity: 50 mL Kit
Substrate: Horseradish Peroxidase
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Product Type: Substrate
Sensitivity: Low picogram

Contents & storage

Upon receipt store at 2-8°C. Product is shipped at ambient temperature.