Miser™ Antibody Extender Solution NC - FAQs

If you are happy with your western signal, you can use the Miser Antibody Extender Solution NC to minimize the amount of antibody you need to use and therefore reduce your costs. If you need to increase sensitivity, then you can use the Pierce Western Blot Signal Enhancer.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The proteins on the blot become more accessible to the antibodies, making it possible for a signal to be produced using less antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The Extender Solution should be used after staining with Ponceau S.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The Extender solution gives consistent, reproducible results on nitrocellulose membranes; results can vary with PVDF membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The Extender Solution should be used after transferring the protein to the membrane and before blocking the membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.