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Thermo Scientific™
Pierce™ ECL Plus Western Blotting Substrate
Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-rayMás información
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| Número de catálogo | DimensionsLxWxH |
|---|---|
| 32132 | |
| 32134 | |
| 32132X3 |
Número de catálogo 32132
Precio (MXN)
-
Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. This product is sold as Pierce ECL 2 Substrate (Cat. #PI80196X3) through Fisher Scientific and other channels.
Features of ECL Plus Substrate:
• Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
• Higher sensitivity—detect targets down to the low-picogram level
• Longer signal duration—sustained light output for as long as 5 hours
• More imaging options—X-ray, CCD or laser-based imagers
• More affordable—high quality and performance at a lower price than other suppliers' similar substrates
Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.
Features of ECL Plus Substrate:
• Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
• Higher sensitivity—detect targets down to the low-picogram level
• Longer signal duration—sustained light output for as long as 5 hours
• More imaging options—X-ray, CCD or laser-based imagers
• More affordable—high quality and performance at a lower price than other suppliers' similar substrates
Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
ConjugadoPeroxidasa de rábano picante (HRP)
DescripciónSustrato para Western Blot Pierce ECL Plus
Contenido del kitReactivo de detección A, 100 ml, Reactivo de detección B, 2,5 ml
SensibilidadPicogramo bajo a femtogramo medio
Suficiente para10 transferencias en minigel; 1000 cm2 de membrana
Método de detecciónQuimioluminiscente
Para utilizar con (aplicación)Biología de proteínas, ensayos y análisis de proteínas, Western Blotting
FormularioLíquido
Membrane CompatibilityNitrocelulosa, PVDF
Línea de productosPierce
Tipo de productoSustrato
CantidadKit de 100 mL
Unit SizeEach
Contenido y almacenamiento
De 2 °C a 8 °C