Thermo Scientific™

Pierce™ ECL Plus Western Blotting Substrate

Catalog number:  32132

 Related applications:

Protein Electrophoresis & Western Blotting

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Thermo Scientific Pierce ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent horseradish peroxidase (HRP) substrate for western blot detection using X-ray film or CCD or laser-based imagers.

Pierce ECL Plus Western Blotting Substrate characteristics include:
Sensitivity: low-picogram to mid-femtogram
Stability: 1-hr working solution stability, 1-year kit stability at 4°C
Compatibility: nitrocellulose and PVDF membranes
Signal duration: up to 5 hours
Recommended primary antibody concentration: 1:1,000–1:20,000 dilution (0.05–1 µg/mL)
Recommended secondary antibody concentration: 1:25,000–1:200,000 dilution (5–40 ng/mL)
Signal: chemiluminescent and fluorescent with absorption/emission maxima of 430/503 nm

Compare and learn more about our western blot chemiluminescent substrates ›
View all HRP substrates available ›

Pierce ECL Plus substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film or CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus substrate an excellent choice for western blot analysis.

This product is sold as Pierce ECL 2 Substrate (Cat. No. PI80196) through Fisher Scientific and other channels.

WARNING: Cancer –
For Research Use Only. Not for use in diagnostic procedures.


Recommended Antibody Concentrations: 2°: 1:25-200K (5-40ng/mL), 1°: 1:1K (0.05- 1µg/mL)
Signal Duration: 5 hr
Detection Method: Chemiluminescent and Fluorescent
Product Line: Pierce™
Product Size: 100 mL
Sensitivity: Low picogram to mid-femtogram
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase) Substrate

Contents & storage

Upon receipt store at 4°C. Product shipped on ice.


Manuals & protocols