Pierce™ ECL Plus Western Blotting Substrate

Catalog number: 32132

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers. This product is sold as Pierce ECL 2 Substrate (Cat. #PI80196) through Fisher Scientific and other channels.

Features of ECL Plus Substrate:

Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
Higher sensitivity—detect targets down to the low-picogram level
Longer signal duration—sustained light output for as long as 5 hours
More imaging options—X-ray, CCD or laser-based imagers
More affordable—high quality and performance at a lower price than other suppliers' similar substrates

Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 100 mL
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Detection Method: Chemiluminescent
Substrate Properties: Chemical Substrate

Contents & storage

Upon receipt store at 4°C. Product shipped on ice.

Documents

Manuals & protocols