Thermo Scientific™

Pierce™ ECL Plus Western Blotting Substrate

Catalog number:  32134

Related applications:

Protein Electrophoresis & Western Blotting

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Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. This product is sold as Pierce ECL 2 Substrate (Cat. #PI80196X3) through Fisher Scientific and other channels.

Features of ECL Plus Substrate:

Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
Higher sensitivity—detect targets down to the low-picogram level
Longer signal duration—sustained light output for as long as 5 hours
More imaging options—X-ray, CCD or laser-based imagers
More affordable—high quality and performance at a lower price than other suppliers' similar substrates

Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.
WARNING: Cancer –
For Research Use Only. Not for use in diagnostic procedures.


Product Line: Pierce™
Recommended Antibody Concentrations: 2°: 1:25-200K (5-40ng/mL), 1°: 1:1K (0.05- 1µg/mL)
Signal Duration: 5 hr
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Detection Method: Chemiluminescent and Fluorescent
Sensitivity: Low picogram to mid-femtogram