Thermo Scientific™

Pierce™ CN/DAB Substrate Kit

Catalog number:  34000

 Related applications:

Cellular Imaging

| Protein Electrophoresis & Western Blotting

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Thermo Scientific Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.

This kit combines two popular chromogenic peroxidase substrate compounds (4-CN and DAB) in a single, stable, 10X stock solution. This CN/DAB solution produces an intense dark black precipitate at sites of bound HRP-conjugated antibodies on probed blots and tissue samples. The Pierce CN/DAB Substrate Kit can be used in Western immunoblotting techniques as well as in dot blotting assays. The kit includes the 10X solution of CN/DAB and accompanying 1X Stable Peroxide Substrate Buffer.

Features of the CN/DAB Substrate Kit:

HRP substrate—for detection of horseradish peroxidase activity on solid media via the combined action of CN (4-chloro-1-naphthol) and DAB (3,3'-diaminobenzidine tetrahydrochloride)
Chromogenic—no special equipment needed for visualization; yields intense black bands that are easy to photograph
Easy-to-use kit—substrate stock solution and stable peroxide substrate buffer mean that there's no dry powders to measure and dissolve
Stable—store refrigerated at 4°C for at least one year

• CN/DAB Solution (10X) and Stable Peroxide Substrate Solution (1X)
WARNING: Reproductive Harm -
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Colorimetric
Product Line: Pierce™
Product Size: 275 mL
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase) Substrate

Contents & storage

Sufficient For: 25 uses at 10 mL each
• CN/DAB (10X), 25 mL
• Stable Peroxide Buffer, 250 mL

Store at 4°C.


Manuals & protocols