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View additional product information for 1-Step™ TMB ELISA Substrate Solutions - FAQs (34029, 34028, 34024, 34022, 34021)
4 product FAQs found
The 1-Step Ultra TMB-ELISA Substrate Solution is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
The 1-Step Ultra TMB-ELISA is our most sensitive substrate for HRP in plate-based assays. It can detect down to ~ 2 pg/well or 20 pg/mL.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
No. This product is formulated to generate a soluble, colored product that absorbs light at 450 nm. When used properly, there will be no precipitate, therefore, this substrate is only appropriate for plate-based assays, such as ELISA.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
No. Although the non-stopped substrate will turn a blue color in the presence of Horseradish Peroxidase and absorb light at 307 nm and 652 nm, color development proceeds very quickly. In the time that it takes to read the full plate, the absorbance of the first wells will have changed. It is better to stop the the color development in all wells at approximately the same time point with an acidic soluton, turning it yellow and then read the absorbance at 450 nm.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.