Thermo Scientific™

BCIP Substrate Powder (5-bromo-4-chloro-3'-indolylphosphate p-toluidine)

Catalog number: 34040
Thermo Scientific™

BCIP Substrate Powder (5-bromo-4-chloro-3'-indolylphosphate p-toluidine)

Catalog number: 34040
Catalog Number
34040
Unit Size
1 g
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
340401 g
-
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Thermo Scientific Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolylphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolylphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).

Features:

AP substrate—for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples
Chromogenic—no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph
Package options—choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor

Highlights of Pierce NBT/BCIP 1-Step Solution (Part No. 34042):
• Best for immunoblotting applications
• Ready-to-use single component
• Sensitive with low background and minimial assay-to-assay variability

Highlights of Pierce NBT/BCIP plus Suppressor 1-Step Solution (Part No. 34070):
• Best for immunohistochemistry applications
• Ready-to-use single component
• Contains 1 mM levamisole to inhibit endogenous phosphatase activity, while yielding an intense dark purple precipitate in the presence of calf intestinal phosphatase

Related Products
1-Step NBT/BCIP Substrate Solution
1-Step NBT/BCIP plus Suppressor Substrate Solution
NBT Substrate Powder (nitro-blue tetrazolium chloride)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Description
Pierce BCIP Substrate
Product Type
Substrate
Detection Method
Colorimetric
Form
Powder
Membrane Compatibility
Nitrocellulose, PVDF
Product Line
Pierce™
Quantity
1 g
Substrate
Alkaline Phosphatase

Contents & Storage

Upon receipt store product at -20°C protected from moisture. Product is shipped at ambient temperature.

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