New Protein Electrophoresis and Western Blotting Support Center—Find technical tips and troubleshooting recommendations from our scientists.

SuperSignal™ West Pico Chemiluminescent Substrate

Catalog number: 34080

Thermo Scientific™  Related applications: Protein Assays and Analysis

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top


Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) HRP substrate for low-picogram-level detection by Western blot analysis.

SuperSignal West Pico Substrate provides intense signal and picogram sensitivity for Western blotting with horseradish peroxidase (HRP) enzyme conjugates. The ECL substrate is compatible with different membranes, blocking reagents and a wide range of antibody dilutions. SuperSignal West Pico Substrate provides excellent performance, versatility and economy for routine Western blotting needs.

Features of SuperSignal West Pico Chemiluminescent Substrate:

ECL—enhanced chemiluminescent substrate for horseradish peroxidase (HRP)
Picogram sensitivity—detect low-picogram amounts of protein in bands on nitrocellulose or PVDF membrane
Good signal duration—incubated blots provide 6 to 8 hours of usable light output when conditions are optimized
Stable reagent—24-hour working solution stability; 1-year kit stability at room temperature
Economical—optimized for dilute antibody concentrations:
• 0.2 to 1.0 µg/mL primary antibody (1:1000 to 1:5000 dilution from 1 mg/mL stock)
• 10 to 50 ng/mL secondary antibody (1:20,000 to 1:100,000 dilution from 1 mg/mL stock)
For Research Use Only. Not for use in diagnostic procedures.


Product Size: 500 mL
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Detection Method: Chemiluminescent
Substrate Properties: Chemical Substrate

Contents & storage

Sufficient For: 60 mini-gel blots; 5000 cm2 of membrane
• Luminol/Enhancer, 250 mL
• Stable Peroxide Buffer, 250 mL

Store at room temperature.


Manuals & protocols