SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Description

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:
• Sensitivity: pictogram to femtogram sensitivity
• Stability: 8-hr working solution stability; 1-year kit stability at room temperature
• Compatibility: nitrocellulose and PVDF membranes
• Signal duration: 6 to 24 hours
• Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 µg/mL)
• Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

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SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.

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Specifications

Product Line:	SuperSignal™
Recommended Antibody Concentrations:	2°: 1:20-100K (10-50ng/mL), 1°: 1:1K (0.2- 1µg/mL)
Shipping Condition:	Room Temperature
Signal Duration:	6-24 hr
Substrate Properties:	Chemical Substrate
Detection Method:	Chemiluminescent
Substrate Type:	HRP (Horseradish Peroxidase) Substrate
Kit Contents:	SuperSignal West Pico PLUS Luminol/Enhancer, 500mL
Quantity:	1 L
Sensitivity:	Low picogram to high femtogram

Contents & storage