Informational:Informational:This is the new, improved version of SuperSignal West Pico Chemiluminescent Substrate (Cat. No. 34080, etc.).
Thermo Scientific™

SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Catalog number: 34578
Thermo Scientific™

SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Catalog number: 34578
Catalog Number
34578
Unit Size
1 L
Price (USD)
516.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
20 mL
Price (USD)
58.50
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
200 mL
Price (USD)
178.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
500 mL
Price (USD)
313.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
345781 L
516.00
Your Price:
***
3457920 mL
58.50
Your Price:
***
34577200 mL
178.00
Your Price:
***
34580500 mL
313.00
Your Price:
***
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Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

SuperSignal West Pico PLUS Chemiluminescent Substrate characteristics include:
Sensitivity: pictogram to femtogram sensitivity
Stability: 8-hr working solution stability; 1-year kit stability at room temperature
Compatibility: nitrocellulose and PVDF membranes
Signal duration: 6 to 24 hours
Recommended primary antibody concentration: 1:1,000–1:5,000 dilution (0.2–1.0 µg/mL)
Recommended secondary antibody concentration: 1:20,000–100,000 dilution (10–50 ng/mL)

Compare and learn more about our western blot chemiluminescent substrates ›
View all HRP substrates available ›

SuperSignal West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions, making it an ideal choice for most western blotting applications.

Specifications

Conjugate
HRP (Horseradish Peroxidase)
Detection Method
Chemiluminescent
Form
Liquid
Product Line
SuperSignal™
Product Type
Substrate
Quantity
1 L
Sensitivity
Low picogram to high femtogram
Substrate
Horseradish Peroxidase
Substrate Properties
Chemical Substrate
Substrate Type
HRP (Horseradish Peroxidase) Substrate
Kit Contents
SuperSignal West Pico PLUS Luminol/Enhancer, 500mL, SuperSignal West Pico PLUS Stable Peroxide, 500mL
Recommended Antibody Concentrations
2°: 1:20-100K (10-50ng/mL), 1°: 1:1K (0.2- 1µg/mL)
Shipping Condition
Room Temperature
Signal Duration
6-24 hr

Contents & Storage

Sufficient for 120 mini-gel blots; 10,000 cm2 of membrane
• SuperSignal West Pico PLUS Luminol/Enhancer, 500 mL
• SuperSignal West Pico PLUS Stable Peroxide, 500 mL

Store at room temperature.

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