Pierce™ Fast Western Blot Kit, ECL Substrate

Catalog number: 35055

Thermo Scientific™  Related applications: Protein Assays and Analysis

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The Thermo Scientific Pierce Fast Western Blot Kit, ECL Substrate contains optimized reagents that shorten the time required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

Features of this kit:

Fast—all the sensitivity of Pierce ECL substrate and saving 4 to 5 hours per blot
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Economical—cost as little as $8 per blot; no expensive consumables or extra equipment
Excellent stability—kit is stable for 1 year stored at 4°C
Easy—complete kit gives you all the components you need to block and probe and develop a blot with your mouse or rabbit primary antibody

The kit provides all the reagents necessary to complete a Western blot being probed with a mouse or rabbit primary antibody. The protocol requires minimal hands-on time and yields results comparable to classic Western blotting with ECL. The included Pierce ECL Substrate produces a chemiluminescent signal, which is detected using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the primary antibody and immunodetection reagents and reprobed.

The Pierce Fast Western Blot Kit is reagent-based system that provides optimized reagents for blocking, antibody dilution and detection of Western blots with Pierce ECL substrate. The protocol is a quick, efficient and economic way to obtain Western blot results without the hassle of buying an instrument and the consumables required by other popular rapid Western blotting systems. No vacuum pump is needed so there are never any clogged lines or membranes that can occur when using instrument-based systems. Because there is no apparatus, there is no need to buy disposable blotting trays that limit the number of blots processed at one time.
For Research Use Only. Not for use in diagnostic procedures.


Substrate: Luminol
Reactivity: Mouse, Rabbit
Product Size: 200 mL
Detection Method: Western Blot

Contents & storage

Sufficient For: 5 mini blots probed with mouse or rabbit primary antibodies
• Fast Western Antibody Diluent, 100 mL
• Fast Western 10X Wash Buffer, 50 mL
• Fast Western Optimized HRP Reagent, 5 mL
• Pierce ECL Detection Reagent 1, 25 mL
• Pierce ECL Detection Reagent 2, 25 mL

Store at 4°C.


Manuals & protocols

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