Thermo Scientific™

Pierce™ Fast Western Blot Kits, SuperSignal™ West Femto, Mouse

Catalog number:  35080

 Related applications:

Protein Electrophoresis & Western Blotting

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The Thermo Scientific Pierce Fast Western Blot Kit, SuperSignal West Femto Substrate contains optimized reagents and protocol for mid-to-low femtogram-level detection and typical Western blot development less than 1 hour after gel transfer.

Features of this kit:

Fast—femtogram levels of detection in about 1 hour
Convenient—no expensive hardware or vacuum required; no clogging issues
Simple—optimized protocol makes Western blot analysis easier than ever
Easy—complete kits contain all components needed to block, probe and develop a blot with your mouse primary antibody

The Fast Western Blot Kit protocol is a quick and efficient method for developing Western blots without the need to purchase expensive instrumentation and consumables required by other rapid Western blotting systems. These complete Pierce Fast Western Blot Kits require minimal hands-on time and yield results comparable to classic Western blotting with SuperSignal West Femto Chemiluminescent Substrate. The kits include SuperSignal West Femto Substrate, the most sensitive enhanced chemiluminescent substrate available for detecting horseradish peroxidase (HRP). This substrate's sensitivity, intensity and duration of the signal allow for easy documentation using photographic or other imaging methods. The developed blots can be repeatedly exposed and stripped of the primary antibody and reprobed. The complete kit contains all the reagents necessary to block and probe a Western blot with a mouse primary antibody.

Related Products
Pierce™ Fast Western Blot Kits, SuperSignal™ West Femto, Rabbit
WARNING: Cancer –
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Western Blot
Product Line: Pierce™, SuperSignal™
Product Size: 400 mL
Reactivity: Mouse
Substrate: Luminol

Contents & storage

Sufficient For: 10 mini blots probed with mouse primary antibodies
• Fast Western Antibody Diluent, 200 mL
• Fast Western 10X Wash Buffer, 100 mL
• Fast Western Mouse Optimized HRP Reagent, Femto, 10 mL
• SuperSignal West Femto Luminol/Enhancer Solution, 50 mL
• SuperSignal West Stable Peroxide Solution, 50 mL

Store kit at 4°C.


Manuals & protocols