3730xl DNA Analyzer (factory refurbished)

Catalog number: 3730XLR

Applied Biosystems™  Related applications: DNA Sequencing | Fragment Analysis | Genotyping & Genomic Profiling | Sanger Sequencing

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The 96-capillary 3730xl DNA Analyzer is the Gold Standard for high throughput genetic analysis. Use this for DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Get the highest quality data at a low cost per sample. This instrument is refurbished by the original manufacturer to meet both manufacturing and product specifications. This system includes the refurbished instrument, new Windows computer, Data Collection software, choice of one analysis software package, installation by an Applied Biosystems “5-Step Certified" Field Service Engineer, chemistry kit, training, new parts and a one year warranty. Availability may be limited.

• Higher optical sensitivity and advanced polymers enable you to obtain higher-quality sequencing data for less.
• Multiple automation features decrease costly human errors.
• Optimized polymers increase your productivity without compromising your results.
• Perform a wide variety of sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, SNP screening and SNP validation.

Large-Scale DNA Analysis on a Small-Scale Budget
By combining advances in automation with innovative optics and proprietary reagents to increase throughput, yield high-quality data, and minimize reagent consumption, the 3730xl Analyzer provides your laboratory and production facility with faster, better, cheaper analysis.

Streamline Your Workflow
The 3730xl DNA Analyzer system is engineered for highly reliable, unattended operation of up to 48 hours (applies to modules with run times longer than 30 minutes). To minimize the need for operator intervention and decrease the risk of human error, automation features include an integrated plate stacker, internal bar code reader, and onboard polymer delivery system.

Increase Productivity
The high signal-to-noise ratio ensures you get high-quality data – even when you use low-concentration samples and reagents. Labor-saving automation features minimize hands-on time and enable you to analyze more data more efficiently.

Get the Highest-Quality Sequencing and Genotyping Data
The enhanced optical design provides a higher signal-to-noise ratio and a more uniform signal profile across the array. This design, combined with our new, advanced polymer, enables the longest read lengths of any available system, and provides enhanced color balance for streamlined genotyping sample handling. In addition, exceptional sensitivity enables higher success rates across a wide range of sample template types and concentrations.

One Instrument, Multiple Applications
With the 3730xl DNA Analyzer, you get the highest-quality data from a wide array of applications. In addition to being ideal for high-throughput sequencing, the analyzer's optimized application assays, instrument, and analysis software also provide a complete solution for genotyping and resequencing.

Integrated Data Analysis Tools Reduce Time-to-Results
The 3730xl DNA Analyzer software suite allows you to generate more meaningful data with less work. This system's labor-saving software suite includes:
• Data Collection (supplied with the instrument): Manages your instrument setup, controls instrument operations, allows real-time data visualization, and performs diagnostics.
• Sequencing Analysis Software: Designed to base-call; assign quality values; trim, display, edit and print DNA sequencing data using the KB basecaller.
• Seqscape®: Provides everything you need to perform resequencing applications such as VariantSEQr™ Resequencing System.
• GeneMapper®: Enables configurable, automated allele calling – a plus for high-throughput genotyping and includes tools for SNPlex™ data analysis

New features include:
• Auto-analysis with GeneMapper® and SeqScape®
• Flexibility to use any choice in dye set option
• Tools to assist with regulatory and compliance requirements (In the United States, this assists with FDA 21CFR part 11).
• Additional optimized run modules covering more applications
• Support for fragment analysis applications on the 96-capillary array

The instrument comes with a one year limited warranty on parts and labor.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


Format: 96-well plate, 384-well plate
Memory: 1 GB  400MHz RAM
Optics: In-capillary detection by dual-side illumination, Multiple Filter Sets: Z, E, G5, G5-RCT, Any4Dyeset, Any5Dyeset and S (optional), Backside-thinned CCD for maximum optical sensitivity
Weight: 180 kg (computer - 30 kg)
Capacity: 16 sample plates (96-well or 384-well)
Dimensions: 100 cm (W) x 73 cm (D) x 89 cm (H)
Throughput: GeneMapper® Software: 4224 samples per day (92,000 genotypes), XLRseq: 768 samples per day (690 Kbases), StdSeq: 2304 samples/day (1550 Kbases), RapidSeq: 3840 samples per day (2100 Kbases), FastSeq: 2304 samples/day (1600 Kbases), LongSeq: 1152 samples/day (980 Kbases)
Performance: XLRseq: 50cm - 900 QV20 bases/3 hours, GeneMapper® Software: 36cm - up to 500 bp res. w/ 0.15 bp sizing precision in 34 min, StdSeq: 36cm - 700 QV20 bases/1 hour, RapidSeq: 36cm - 550 QV20 bases/36 min, FastSeq: 50cm - 700 QV20 bases/1 hour, LongSeq: 50cm - 850 QV20 bases/2 hours
Read Length: 400 - 900 bases (varies depending on run module)
Product Size: 1 system
Plan Duration: 1 Year Limited Warranty on Parts and Labor
Sample Volume: 10-50 µl for 96-well plate, 5-30 µl for 384-well plate
Plate Handling: Plate Seal: Automatic on-board piercing (septa or polypropylene heat seal), Integrated auto sampler and plate stacker, Internal bar code reader
Capillary Length: 36 cm or 50 cm
Operating System: Microsoft Windows 7
Reagent Handling: On-board reagents for up to 48 hrs unattended operation (with certain run modules), Run modules: 5 sequencing, 1 fragment analysis and optional SNPlex™, Automated polymer replenishment pump for increased throughput
External Computer: Computer and Flat Panel Monitor
Power Requirements: Voltage: 200-220 V or 230-240 V ±10%, Current: 15 amps max. Requires 30 amp receptacle w/in 3 m (10 ft) of instrument back., Power Dissipated: About 2000 watts
Compatible Polymers: POP Conformational Analysis Polymer, POP-7
Polymer Consumption: 50 cm: 250µl⁄run, 36 cm: 200µl⁄run
Number of Capillaries: 96 Capillaries
Operating Environment: Temperature: 18°C-30°C, Humidity: 20%-80% (non-condensing)
For Use With (Equipment): 3730xl DNA Analyzer
Temperature Range (Metric): Temperature-controlled oven (18-70°C range) and detection cell heater
High Throughput Compatibility: High Throughput-Compatible

Contents & storage

This system includes a factory refurbished 96 capillary instrument, a new computer with Windows, data collection software, choice of one analysis software package, and chemistry kit.


Manuals & protocols