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Pierce™ Protein-Free Blocking Buffer
Pierce™ Protein-Free Blocking Buffer
Thermo Scientific™

Pierce™ Protein-Free Blocking Buffer

Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background.
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Catalog NumberQuantityChemical Name or Material
375721 LProtein-Free (PBS)
37584100 mLProtein-Free (PBS)
37585100 mLProtein-Free (TBS)
375701 LProtein-Free (TBS)
375731 LProtein-Free T20 (PBS)
375711 LProtein-Free T20 (TBS)
Catalog number 37572
Price (USD)
244.65
Online Exclusive
275.00
Save 30.35 (11%)
Each
Add to cart
Quantity:
1 L
Chemical Name or Material:
Protein-Free (PBS)
Request bulk or custom format
Price (USD)
244.65
Online Exclusive
275.00
Save 30.35 (11%)
Each
Add to cart
Pierce Protein-Free Blocking Buffer is a non-protein compound that provides effective blocking for membrane-based and plate-based protein detection methods, resulting in extremely low background. It is supplied in a convenient, ready-to-use format.

Features of Protein-Free Blocking Buffer include:

  • Protein-free blocker—minimizes or eliminates cross-reactivity associated with protein-based blocking buffers
  • Applications—compatible with western blotting (membranes), ELISA (microplates), and arrays (coated glass slides)
  • Streptavidin-friendly—free of biotin; no interference with avidin-biotin detection systems
  • High-performance—optimized and validated in many protein methods to provide high signal-to-noise ratio (i.e., no quenching of specific binding and signal but eliminating nonspecific binding and background)

Traditional blocking buffers contain proteins that can cross-react in certain immunodetection systems, resulting in high background and reduced signal. Pierce Protein-Free Blocking Buffers are devoid of protein while remaining highly effective at blocking plates, membranes, and other surfaces for ELISA, western blotting, glass slide arrays, and other applications.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or MaterialProtein-Free (PBS)
Recommended StorageUpon receipt store product at room temperature. After opening, store product at 4°C.
Concentration1X
For Use With (Application)ELISA
Physical FormLiquid
Product LinePierce
Quantity1 L
Unit SizeEach

Frequently asked questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Is the Pierce Protein-Free Blocking Buffer (Cat. No. 37571) animal-origin free (AOF)?

Yes, it is animal-origin free (AOF).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.