Thermo Scientific™

1-Step™ Ultra TMB-Blotting Solution

Catalog number:  37574

 Related applications:

Protein Electrophoresis & Western Blotting

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Thermo Scientific 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting.

This precipitating, colorimetric Western blot substrate for HRP provides high sensitivity, increased signal-to-noise ratio and low background compared to many other chromogenic substrates. The blotting solution contains soluble TMB (3,3',5,5'-tetramethylbenzidine), which reacts very quickly with horseradish peroxidase enzyme to produce an insoluble dark blue precipitate that does not fade or flake. The substrate is compatible with both nitrocellulose and PVDF membranes. The blotting solution is supplied ready to use with no mixing required.

Features of 1-Step Ultra TMB-Blotting Solution:

Fast—protein bands visible in less than one minute
Fade-resistant—protein bands stable after membrane drying
Sensitive—detection limit similar to Pierce ECL Western Blotting Substrate
Chromogenic—no special equipment needed for visualization; produces dark blue bands
Ready to use—no organic solvents are required to dissolve; no dilution necessary for use

• Chromogenic Western blot detection
• Nitrocellulose membrane blots
• PVDF membrane blots
For Research Use Only. Not for use in diagnostic procedures.


Detection Method: Colorimetric
Product Line: 1-Step™
Product Size: 250 mL
Substrate Properties: Chemical Substrate
Substrate Type: HRP (Horseradish Peroxidase) Substrate

Contents & storage

Upon receipt store product at 4°C. Product is shipped at ambient temperature.


Manuals & protocols