The IEF Marker 3-10 is a ready-to-use, unstained protein marker especially developed for IEF applications. It contains 13 purified proteins ranging in pI from 3-10. It works well on horizontal and vertical one-dimensional IEF gels and can be used for both native and denaturing conditions (e.g., 8 M urea, to determine the isoelectric point (pI) of unknown protein samples. Here are the pIs and origins of the proteins in the IEF Marker, 3-10:
- Band no. 9: Cytochrome C; Pig heart origin; 10.7 pI
- Band no. 8 Ribonuclease A; Bovine pancreas origin; 9.5 pI
- Band no. 7 c, m, a Lectin; Lens culinaris origin; 8.3, 8.0, 7.8 pI
- Band no. 6 c, a Myoglobin; Horse muscle origin; 7.4, 6.9 pI
- Band no. 5 Carbonic anhydrase; Bovine erythrocytes origin; 6.0 pI
- Band no. 4 c, a B-Lactoglobulin; Bovine milk origin; 5.3, 5.2 pI
- Band no. 3 Trypsin inhibitor; Soybean origin; 4.5 pI
- Band no. 2 Glucose oxidase; Aspergillus niger origin; 4.2 pI
- Band no. 1 Amyloglucosidase; Aspergillus niger origin; 3.5 pI
Note: Temperature = 5 degrees C for pI values given. pI is temperature dependent, i.e. decreases as temperature increases (approximately -0.005 to-0.03 pH units per degree C)
c = cathodic; m = middle; a = anodic
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