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Gibco™
DMEM/F-12, HEPES, powder
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of詳細を見る
| 製品番号（カタログ番号） | 数量 |
|---|---|
| 42400010 または、製品番号42400-010 | 1 L |
製品番号（カタログ番号） 42400010
または、製品番号42400-010
価格（JPY）
-
数量:
1 L
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM / F-12 is modified as follows:
| With | Without |
| • L-glutamine | • Sodium Bicarbonate |
| • HEPES | |
| • Phenol Red |
The complete formulation is available.Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.
DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
仕様
培養環境CO2
製造品質cGMP-compliant under the ISO 13485 standard
浸透圧260 - 300 mOsm/kg
製品ラインGibco
製品タイプDulbecco's Modified Eagle Medium (DMEM)
数量1 L
品質保持期間36 Months
出荷条件Room Temperature
分類Animal Origin-free
Culture TypeMammalian Cell Culture
形状Powder
Serum LevelStandard Serum Supplementation
添加剤ありGlutamine, HEPES, Phenol Red, Sodium Pyruvate
添加剤なしNo Sodium Bicarbonate
pH5.6 to 6
Unit SizeEach
組成および保存条件
Storage conditions: 2-8° C.
Shipping conditions: Ambient.
Shelf life: 36 months from date of manufacture.
Shipping conditions: Ambient.
Shelf life: 36 months from date of manufacture.